DHFR Substrates

Santa Cruz Biotechnology now offers a broad range of DHFR Substrates for use in various applications. Dihydrofolate reductase (DHFR) is a key enzyme involved in the folate pathway, playing a critical role in the synthesis of nucleotides and the maintenance of cellular DNA and RNA synthesis. DHFR Substrates are essential tools in scientific research, enabling the detailed study of DHFR enzyme activity, its role in cellular metabolism, and the impact of its inhibition on nucleotide biosynthesis. Researchers utilize these substrates to explore how DHFR contributes to the reduction of dihydrofolate to tetrahydrofolate, a reaction that is crucial for the regeneration of methionine and the synthesis of thymidylate, both of which are vital for DNA replication and repair. These substrates are widely employed in enzymatic assays to measure DHFR activity, investigate enzyme kinetics, and understand how different factors, including mutations and inhibitors, affect DHFR function. Additionally, DHFR Substrates are valuable in studies focused on understanding the broader implications of folate metabolism in cell proliferation, particularly in rapidly dividing cells such as those found in tumors or in immune responses. The availability of these substrates has significantly advanced research in fields such as biochemistry, molecular biology, and genetics, providing researchers with essential tools to dissect the complex interactions within the folate pathway and their implications for cellular growth and function. View detailed information on our available DHFR Substrates by clicking on the product name.