CPSF4 Antibody (F-8): sc-514752

CPSF4 Antibody (F-8) is a mouse monoclonal IgG2b antibody that detects CPSF4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPSF4, also known as Cleavage and polyadenylation specificity factor subunit 4, is a crucial nuclear protein that is part of the CPSF complex, which is essential for the proper processing of pre-mRNA, particularly in the formation of the 3′-end. This protein contains five C3H1-type zinc fingers and one CCHC-type zinc finger, which are important for structural integrity and function. The CPSF complex, composed of multiple subunits, recognizes the AAUAAA signal in pre-mRNA, facilitating RNA cleavage and poly(A) tail synthesis. Notably, CPSF4 interacts with other proteins, such as the suppressor of forked subunit of the cleavage stimulatory factor and poly(A) polymerase, to enhance polyadenylation efficiency. Additionally, CPSF4 interaction with U1 snRNP-A stabilizes binding to AAUAAA-containing RNAs, further underscoring CPSF4′s importance in gene expression regulation. anti-CPSF4 antibody (F-8) is an invaluable tool for researchers studying RNA processing and gene regulation mechanisms, providing insights into molecular pathways that govern mRNA stability and translation.

The preferred CPSF4 antibody is CPSF4 Antibody (A-11): sc-393316.