CPSF4 Antibody (A-11): sc-393316
- CPSF4 Antibody (A-11) is a mouse monoclonal IgG1 κ CPSF4 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-102 mapping at the N-terminus of CPSF4 of human origin
- CPSF4 Antibody (A-11) is recommended for detection of CPSF4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CPSF4 Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CPSF4 Antibody (A-11) for WB applications. These reagents are now offered in bundles with CPSF4 Antibody (A-11) (see ordering information below).
CPSF4 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPSF4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPSF4 (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPSF4 protein, also known as cleavage and polyadenylation specificity factor subunit 4 or NS1 effector domain-binding protein 1, is a crucial nuclear protein that is part of the CPSF4/YTH1 family and features five C3H1-type zinc fingers along with one CCHC-type zinc finger. CPSF4 plays an essential role in the cleavage and polyadenylation specificity factor (CPSF) complex, which is vital for the proper processing of pre-mRNA 3′-ends. CPSF is a multisubunit complex that recognizes the AAUAAA signal in pre-mRNA, facilitating RNA cleavage and poly(A) synthesis through interactions with other proteins. CPSF4 interacts with the snRNP-A protein (U1 snRNP-A), which enhances the stability of CPSF′s interaction with AAUAAA-containing RNAs, thereby increasing the efficiency of polyadenylation. The precise processing of the 3′ core poly(A) site is also dependent on specific sequences located 76 nucleotides upstream of the AAUAAA hexamer, underscoring the importance of CPSF4 in gene expression regulation and mRNA stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CPSF4 Antibody (A-11) References:
- Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. | de Vries, H., et al. 2000. EMBO J. 19: 5895-904. PMID: 11060040
- Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. | Kaufmann, I., et al. 2004. EMBO J. 23: 616-26. PMID: 14749727
- Transcriptome analysis of human gastric cancer. | Oh, JH., et al. 2005. Mamm Genome. 16: 942-54. PMID: 16341674
- The H5N1 influenza virus NS genes selected after 1998 enhance virus replication in mammalian cells. | Twu, KY., et al. 2007. J Virol. 81: 8112-21. PMID: 17522219
- Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor. | Jenny, A. and Keller, W. 1995. Nucleic Acids Res. 23: 2629-35. PMID: 7651824
- The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. | Barabino, SM., et al. 1997. Genes Dev. 11: 1703-16. PMID: 9224719
- Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF. | Salinas, CA., et al. 1998. Mol Gen Genet. 257: 672-80. PMID: 9604891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF4 Antibody (A-11) | sc-393316 | 200 µg/ml | $322.00 | |||
CPSF4 Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-527679 | 200 µg Ab; 10 µg BP | $361.00 | |||
CPSF4 Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-533052 | 200 µg Ab; 20 µg BP | $361.00 | |||
CPSF4 Antibody (A-11) AC | sc-393316 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CPSF4 Antibody (A-11) HRP | sc-393316 HRP | 200 µg/ml | $322.00 | |||
CPSF4 Antibody (A-11) FITC | sc-393316 FITC | 200 µg/ml | $336.00 | |||
CPSF4 Antibody (A-11) PE | sc-393316 PE | 200 µg/ml | $349.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 488 | sc-393316 AF488 | 200 µg/ml | $364.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 546 | sc-393316 AF546 | 200 µg/ml | $364.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 594 | sc-393316 AF594 | 200 µg/ml | $364.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 647 | sc-393316 AF647 | 200 µg/ml | $364.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 680 | sc-393316 AF680 | 200 µg/ml | $364.00 | |||
CPSF4 Antibody (A-11) Alexa Fluor® 790 | sc-393316 AF790 | 200 µg/ml | $364.00 |