Cdc27 Antibody (C-4): sc-13154

Cdc27 Antibody (C-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdc27 of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Cdc27 monoclonal antibody (C-4) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Cdc27 plays a crucial role in the regulation of the cell cycle by being a key component of the anaphase-promoting complex (APC), which is responsible for the ubiquitination and subsequent degradation of cyclin B. This degradation is essential for the transition from metaphase to anaphase during cell division, ensuring proper chromosome segregation and maintaining genomic stability. Dysregulation of Cdc27 and the APC can lead to uncontrolled cell proliferation and has been implicated in various cancers, highlighting the importance of Cdc27 in cell cycle regulation and its potential as a therapeutic target.

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Cdc27 Antibody (C-4) References:

1. Downregulation of CDC27 inhibits the proliferation of colorectal cancer cells via the accumulation of p21Cip1/Waf1.  |  Qiu, L., et al. 2016. Cell Death Dis. 7: e2074. PMID: 26821069
2. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27.  |  Link, LA., et al. 2016. Mol Cancer Res. 14: 634-46. PMID: 27102006
3. CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and Metastasis via Twist-Induced Epithelial-Mesenchymal Transition.  |  Xin, Y., et al. 2018. Cell Physiol Biochem. 50: 501-511. PMID: 30308498
4. CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma.  |  Song, Y., et al. 2020. Front Oncol. 10: 488. PMID: 32391258
5. The importance of CDC27 in cancer: molecular pathology and clinical aspects.  |  Kazemi-Sefat, GE., et al. 2021. Cancer Cell Int. 21: 160. PMID: 33750395
6. CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.  |  Qiu, L., et al. 2022. Front Oncol. 12: 774458. PMID: 35242701
7. A Novel Gene CDC27 Causes SLE and Is Associated With the Disease Activity.  |  Shang, S., et al. 2022. Front Immunol. 13: 876963. PMID: 35418986
8. LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway.  |  Qi, F., et al. 2023. Epigenetics. 18: 2195305. PMID: 36994860
9. Functional and Genetic Analyses Unveil the Implication of CDC27 in Hemifacial Microsomia.  |  Song, W., et al. 2024. Int J Mol Sci. 25: PMID: 38731925
10. CDC27 gene expression patterns as a potential biomarker in Acute Leukemia.  |  Pouriafar, Y., et al. 2024. Mol Biol Rep. 51: 865. PMID: 39073611

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc27 Antibody (C-4) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc27 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc27 Antibody (C-4) Specifications

Alternate Product Name: Cdc27

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: Cdc27

Epitope Species: human

Product Reactivity/Detection: Cdc27

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for Cdc27 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cdc27 antibody is Cdc27 Antibody (AF3.1): sc-9972.