Cdc27 Antibody (AF3.1) recommended for detection of Cdc27 of mouse, rat, human and x origin by WB, IP, IF and IHC(P)

Cdc27 Antibody (AF3.1): sc-9972

Cdc27 Antibody (AF3.1) is rated 5.0 out of 5 by 11.
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Datasheets

    • Anti-Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b (kappa light chain) Cdc27 antibody provided at 200 µg/ml
    • raised against amino acids 814-823 of Cdc27 of human origin
    • Cdc27 Antibody (AF3.1) is recommended for detection of Cdc27 of mouse, rat, human and x origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine and canine
    • Anti-Cdc27 Antibody (AF3.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc27 (AF3.1): sc-9972.
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Can you recommend a positive control to use with Cdc27 (AF3.1): sc-9972 for Western blot?

Asked by: jenni
We recommend K-562 (sc-2203), RAW 264.7 (sc-2211) or HeLa (sc-2200) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rab 7 in these cell lines.
Answered by: Technical Support 13
Date published: 2017-01-20

How can Cdc27 (AF3.1): sc-9972 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Cdc27 (AF3.1): sc-9972 monoclonal antibody is currently available conjugated to phycoerythrin (sc-9972 PE), fluorescein (sc-9972 FITC), Alexa Fluor® 488 (sc-9972 AF488) or Alexa Fluor® 647 (sc-9972 AF647)
Answered by: Technical Support 2
Date published: 2017-01-16
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Rated 5 out of 5 by from good product It is a good antibody and works well for western blot.
Date published: 2019-10-28
Rated 5 out of 5 by from good product It is a good antibody and works well for western blot.
Date published: 2018-08-22
Rated 5 out of 5 by from Outstanding Very strong western blot data; also superb IF and IHC staining
Date published: 2017-01-25
Rated 5 out of 5 by from Great IF staining We used this antibody for IF staining and got great images and nice intensity form HeLa cells nucleus
Date published: 2017-01-18
Rated 5 out of 5 by from Works well in IP & WB Works well in IP & WB in detecting endogenous CDC27 in HeLa cells. The rest of the APC/C co-IPs with CDC27.
Date published: 2016-12-06
Rated 5 out of 5 by from This antibody worked well in out blot in This antibody worked well in out blot in HeLa cells.
Date published: 2015-06-05
Rated 5 out of 5 by from Good WB antibody in rat cerebellar granule neurons Good WB antibody in rat cerebellar granule neurons. -SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Worked well in WB in human transfected HeLa cells Worked well in WB in human transfected HeLa cells. -SCBT Publication Review
Date published: 2015-04-15
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