caspase-8 p18 Antibody (D-8): sc-5263

Caspase-8 p18 Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects the p18 subunit and precursor of caspase-8 in mouse, rat, and human samples through western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. caspase-8 p18 (D-8) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. caspase-8 plays a crucial role in the apoptotic pathway, acting as an initiator caspase that activates downstream effector caspases by cleaving their inactive precursors. This activation cascade is vital for programmed cell death, particularly in response to signals from tumor necrosis factor receptor superfamily members such as TNFRSF1A and TNFRSF6/FAS. caspase-8 forms a complex with the adaptor molecule FADD, known as the death-inducing signaling complex (DISC), which is essential for activation. Once activated, caspase-8 dissociates from the DISC and further propagates the apoptotic signal by activating other caspases. The heterodimeric structure of caspase-8, consisting of the p18 and p10 subunits, is predominantly expressed in peripheral blood leukocytes, thymus, liver, and spleen, highlighting importance in immune regulation. Mutations in the CASP8 gene, which encodes for caspase-8, can lead to caspase-8 deficiency disorder (CASP8D), a condition characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, potentially resulting in immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes.

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caspase-8 p18 Antibody (D-8) References:

1. Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction.  |  Wesselborg, S., et al. 1999. Blood. 93: 3053-63. PMID: 10216102
2. Involvement of FADD and caspase-8 signalling in detachment-induced apoptosis.  |  Rytömaa, M., et al. 1999. Curr Biol. 9: 1043-6. PMID: 10508619
3. The atomic-resolution structure of human caspase-8, a key activator of apoptosis.  |  Watt, W., et al. 1999. Structure. 7: 1135-43. PMID: 10508785
4. Pro-caspase-8 is predominantly localized in mitochondria and released into cytoplasm upon apoptotic stimulation.  |  Qin, ZH., et al. 2001. J Biol Chem. 276: 8079-86. PMID: 11102441
5. Caspase 8 activation independent of Fas (CD95/APO-1) signaling may mediate killing of B-chronic lymphocytic leukemia cells by cytotoxic drugs or gamma radiation.  |  Jones, DT., et al. 2001. Blood. 98: 2800-7. PMID: 11675354
6. p38-MAPK signals survival by phosphorylation of caspase-8 and caspase-3 in human neutrophils.  |  Alvarado-Kristensson, M., et al. 2004. J Exp Med. 199: 449-58. PMID: 14970175
7. Proteasomal regulation of caspase-8 in cancer cell apoptosis.  |  Fiandalo, MV., et al. 2013. Apoptosis. 18: 766-76. PMID: 23456622
8. The Fas death factor.  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
9. Contenders in FasL/TNF death signaling.  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
10. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
11. FLICE is activated by association with the CD95 death-inducing signaling complex (DISC).  |  Medema, JP., et al. 1997. EMBO J. 16: 2794-804. PMID: 9184224
12. Bcl-xL functions downstream of caspase-8 to inhibit Fas- and tumor necrosis factor receptor 1-induced apoptosis of MCF7 breast carcinoma cells.  |  Srinivasan, A., et al. 1998. J Biol Chem. 273: 4523-9. PMID: 9468507

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-8 p18 Antibody (D-8) has 103 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-8 p18 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-8 p18 Antibody (D-8) Specifications

Alternate Product Name: CASP8

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 217-350

Epitope Target: caspase-8 p18

Epitope Species: human

Product Reactivity/Detection: p18 subunit and precursor of caspase-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 103 citations in various scientific publications

Available Conjugates/Forms for caspase-8 p18 Antibody (D-8): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790