caspase-8 Antibody (4.1.20): sc-73526

Caspase-8 Antibody (4.1.20) is a mouse monoclonal IgG1 antibody that detects caspase-8 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as the most upstream protease in the cascade initiated by tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and mediated by TNFRSF6/FAS. Upon activation, caspase-8 cleaves and activates downstream effector caspases, leading to programmed cell death, which is essential for maintaining cellular homeostasis and eliminating damaged or infected cells. Caspase-8 is primarily located in the cytoplasm, where caspase-8 forms a complex with the adaptor molecule FADD, creating the death-inducing signaling complex (DISC) that is pivotal for activation. Proper functioning of caspase-8 is vital, as defects in the CASP8 gene can result in caspase-8 deficiency disorder (CASP8D), characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, ultimately leading to immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes. Anti-caspase-8 antibody (4.1.20) is an invaluable tool for researchers studying apoptosis and its implications in various diseases, including cancer and autoimmune disorders.

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.