caspase-8 Antibody (3C121): sc-70501
- caspase-8 Antibody (3C121) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
- raised against recombinant full length human caspase-8 protein
- recommended for detection of caspase-8 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- See caspase-8 (8CSP03): sc-56070 for caspase-8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for caspase-8 Antibody (3C121) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-8 Antibody (3C121) (see ordering information below).
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Caspase-8 Antibody (3C121) is a mouse monoclonal IgG1 antibody that detects caspase-8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as the most upstream protease in the activation cascade triggered by tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and Fas (TNFRSF6). Upon binding to the adaptor molecule FADD, caspase-8 forms the death-inducing signaling complex (DISC), which is essential for its activation. The active caspase-8, a heterodimer composed of p18 and p10 subunits, is then released from the DISC to activate downstream effector caspases, thereby propagating the apoptotic signal. This process is vital for maintaining cellular homeostasis and eliminating damaged or unwanted cells, and defects in the CASP8 gene can lead to caspase-8 deficiency disorder (CASP8D), which is associated with severe immunodeficiency and splenomegaly due to impaired T lymphocyte, natural killer cell, and B lymphocyte activation. Anti-caspase-8 antibody (3C121) is an invaluable tool for researchers studying apoptosis and its implications in various diseases, including cancer and autoimmune disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
caspase-8 Antibody (3C121) References:
- Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption. | Li, M., et al. 2022. Dev Cell. 57: 2365-2380.e8. PMID: 36243012
- Reduced graphene oxide quenched peptide probe for caspase-8 activity detection and cellular imaging. | Tao, X., et al. 2022. Mikrochim Acta. 189: 463. PMID: 36418555
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-8 Antibody (3C121) | sc-70501 | 200 µg/ml | $322.00 | |||
caspase-8 Antibody (3C121): m-IgG Fc BP-HRP Bundle | sc-539284 | 200 µg Ab; 10 µg BP | $361.00 | |||
caspase-8 Antibody (3C121): m-IgG1 BP-HRP Bundle | sc-541378 | 200 µg Ab; 20 µg BP | $361.00 |