caspase-8 Antibody (2.1.24): sc-81657

Caspase-8 Antibody (2.1.24) is a mouse monoclonal IgG1 antibody that detects caspase-8 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as the most upstream protease in the cascade initiated by tumor necrosis factor receptor superfamily members, such as TNFRSF1A and TNFRSF6/FAS. Upon activation, caspase-8 cleaves and activates downstream effector caspases, leading to the execution of apoptosis, a vital process for maintaining cellular homeostasis and eliminating damaged or unwanted cells. Caspase-8 is primarily located in the cytoplasm, where caspase-8 forms a complex with the adaptor molecule FADD, creating the death-inducing signaling complex (DISC) that is essential for activation. Proper functioning of caspase-8 is critical, as defects in the CASP8 gene can result in caspase-8 deficiency disorder (CASP8D), which is characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, ultimately leading to immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes. Anti-caspase-8 antibody (2.1.24) is an invaluable tool for researchers studying apoptosis and its implications in various diseases, including cancer and autoimmune disorders.

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.