caspase-7 Antibody (7CSP03): sc-56066

caspase-7 Antibody (7CSP03) is a mouse monoclonal IgG1 antibody that detects caspase-7 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Caspase-7 is a crucial member of the Ced-3/caspase-1 family of cysteine proteases, which are integral to the apoptotic process, facilitating programmed cell death by cleaving specific target proteins that regulate cellular longevity. Notably, caspase-7 cleaves poly(ADP-ribose) polymerase (PARP), a nuclear protein that plays a vital role in DNA repair and cellular stress responses. PARP cleavage by caspase-7 generates an apoptotic fragment, which is a hallmark of apoptosis and signifies a cell′s commitment to programmed cell death. This function is particularly important in cancer and neurodegenerative diseases, where apoptosis regulation is often disrupted. Anti-caspase-7 antibody (7CSP03) enables researchers to gain insights into apoptosis mechanisms and caspase-7′s role in various biological processes, making this antibody an invaluable tool for studies in cell biology and disease research.

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caspase-7 Antibody (7CSP03) References:

1. Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulation.  |  Young, JE., et al. 2007. J Biol Chem. 282: 30150-60. PMID: 17646170
2. Executioner caspase-3 and caspase-7 are functionally distinct proteases.  |  Walsh, JG., et al. 2008. Proc Natl Acad Sci U S A. 105: 12815-9. PMID: 18723680
3. Assessment of apoptosis by immunohistochemistry to active caspase-3, active caspase-7, or cleaved PARP in monolayer cells and spheroid and subcutaneous xenografts of human carcinoma.  |  Bressenot, A., et al. 2009. J Histochem Cytochem. 57: 289-300. PMID: 19029405
4. Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes.  |  Edelmann, B., et al. 2011. EMBO J. 30: 379-94. PMID: 21157428
5. Non-apoptotic role for caspase-7 in hair follicles and the surrounding tissue.  |  Vesela, B., et al. 2015. J Mol Histol. 46: 443-55. PMID: 26253163
6. Low cleaved caspase-7 levels indicate unfavourable outcome across all breast cancers.  |  Lindner, AU., et al. 2018. J Mol Med (Berl). 96: 1025-1037. PMID: 30069746
7. miR-224 Is Significantly Upregulated and Targets Caspase-3 and Caspase-7 During Colorectal Carcinogenesis.  |  Fassan, M., et al. 2019. Transl Oncol. 12: 282-291. PMID: 30448733
8. Mcl1 protein levels and Caspase-7 executioner protease control axial organizer cells survival.  |  Sena, E., et al. 2020. Dev Dyn. 249: 847-866. PMID: 32141178
9. Exoenzyme Y induces extracellular active caspase-7 accumulation independent from apoptosis: modulation of transmissible cytotoxicity.  |  Renema, P., et al. 2020. Am J Physiol Lung Cell Mol Physiol. 319: L380-L390. PMID: 32579398
10. SALIS transcriptionally represses IGFBP3/Caspase-7-mediated apoptosis by associating with STAT5A to promote hepatocellular carcinoma.  |  Liu, X., et al. 2022. Cell Death Dis. 13: 642. PMID: 35871161

The preferred caspase-7 antibody is caspase-7 Antibody (10-1-62): sc-56063.