caspase-7 Antibody (10-1-62): sc-56063

caspase-7 Antibody (10-1-62) is a mouse monoclonal IgG1 kappa light chain antibody that detects caspase-7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). caspase-7 Antibody (10-1-62) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-7, also known as Mch3, ICE-LAP3, or CMH-1, is a crucial member of the Ced-3/caspase-1 family of cysteine proteases, which are integral to the apoptotic process. This family is characterized by unique sequence, structure, and substrate specificity, distinguishing caspase-7 from other protease families. Caspase-7 plays a vital role in apoptosis by cleaving specific target proteins essential for cellular survival, including poly(ADP-ribose) polymerase (PARP), which is involved in DNA repair and cellular stress responses. PARP cleavage by caspase-7 leads to the generation of an apoptotic fragment, facilitating the progression of programmed cell death. Caspase-7 is activated by upstream caspases, such as caspase-10, in response to apoptotic signals, highlighting caspase-7′s importance in the regulation of apoptosis and cellular homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-7 Antibody (10-1-62) References:

1. Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7.  |  Germain, M., et al. 1999. J Biol Chem. 274: 28379-84. PMID: 10497198
2. Yeast two-hybrid screening using constitutive-active caspase-7 as bait in the identification of PA28gamma as an effector caspase substrate.  |  Araya, R., et al. 2002. Cell Death Differ. 9: 322-8. PMID: 11859414
3. Inactivating mutations of CASPASE-7 gene in human cancers.  |  Soung, YH., et al. 2003. Oncogene. 22: 8048-52. PMID: 12970753
4. Caspase-7 gene disruption reveals an involvement of the enzyme during the early stages of apoptosis.  |  Korfali, N., et al. 2004. J Biol Chem. 279: 1030-9. PMID: 14583630
5. Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis.  |  Brentnall, M., et al. 2013. BMC Cell Biol. 14: 32. PMID: 23834359
6. Characterization of caspase-7 interaction with RNA.  |  Desroches, A. and Denault, JB. 2021. Biochem J. 478: 2681-2696. PMID: 34156061
7. Caspase-7 activates ASM to repair gasdermin and perforin pores.  |  Nozaki, K., et al. 2022. Nature. 606: 960-967. PMID: 35705808
8. Apoptotic caspase-7 activation inhibits non-canonical pyroptosis by GSDMB cleavage.  |  Li, X., et al. 2023. Cell Death Differ. 30: 2120-2134. PMID: 37591921
9. Chromosomal localization of the human genes, CPP32, Mch2, Mch3, and Ich-1, involved in cellular apoptosis.  |  Tiso, N., et al. 1996. Biochem Biophys Res Commun. 225: 983-9. PMID: 8780721
10. Caspases: the executioners of apoptosis.  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844
11. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver.  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
12. Signaling pathway activated during apoptosis of the prostate cancer cell line LNCaP: overexpression of caspase-7 as a new gene therapy strategy for prostate cancer.  |  Marcelli, M., et al. 1999. Cancer Res. 59: 382-90. PMID: 9927051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-7 Antibody (10-1-62) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-7 Antibody (10-1-62) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-7 Antibody (10-1-62) Specifications

Alternate Product Name: caspase-7

Product Clone Name: 10-1-62

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: caspase-7

Epitope Species: human

Product Reactivity/Detection: caspase-7, recombinant caspase-7 as well as full length procaspase-7 and processed enzyme

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for caspase-7 Antibody (10-1-62): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin