caspase-6 p10 Antibody (H-12): sc-377393

Caspase-6 p10 Antibody (H-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects the p10 subunit and precursor of caspase-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-caspase-6 antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-6 is a member of the Ced-3/caspase-1 family of cysteine proteases, which are crucial for executing apoptosis, a programmed cell death process essential for maintaining cellular homeostasis and development. The p10 subunit of caspase-6 plays a significant role in cleaving specific target proteins, including nuclear lamins, which are vital for preserving nuclear envelope structural integrity and overall cellular morphology. This cleavage activity facilitates cellular component disassembly during apoptosis, allowing for orderly removal of dying cells. Caspase-6 interacts with other caspases, such as caspase-3 and caspase-7, amplifying apoptotic signaling cascade in response to cellular stress or damage. Human caspase-6 exists in two isoforms, with p10 variant potentially undergoing phosphorylation at Ser 257, which may influence activity and interactions within apoptotic pathway.

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caspase-6 p10 Antibody (H-12) References:

1. Caspase-6 role in apoptosis of human neurons, amyloidogenesis, and Alzheimer's disease.  |  LeBlanc, A., et al. 1999. J Biol Chem. 274: 23426-36. PMID: 10438520
2. Caspase-6 is the direct activator of caspase-8 in the cytochrome c-induced apoptosis pathway: absolute requirement for removal of caspase-6 prodomain.  |  Cowling, V. and Downward, J. 2002. Cell Death Differ. 9: 1046-56. PMID: 12232792
3. Caspase-6 activation in familial alzheimer disease brains carrying amyloid precursor protein or presenilin i or presenilin II mutations.  |  Albrecht, S., et al. 2009. J Neuropathol Exp Neurol. 68: 1282-93. PMID: 19915487
4. In vitro proteolysis of myofibrillar proteins from beef skeletal muscle by caspase-3 and caspase-6.  |  Huang, F., et al. 2011. J Agric Food Chem. 59: 9658-63. PMID: 21806076
5. Spinal Caspase-6 Contributes to Intrathecal Morphine-induced Acute Itch and Contact Dermatitis-induced Chronic Itch Through Regulating the Phosphorylation of Protein Kinase Mζ in Mice.  |  Pei, X., et al. 2024. Neuroscience. 539: 21-34. PMID: 38176610
6. Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
7. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
8. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death.  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
9. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
10. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
11. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
12. The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex.  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-6 p10 Antibody (H-12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-6 p10 Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-6 p10 Antibody (H-12) Specifications

Alternate Product Name: caspase-6 p10

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 194-253

Epitope Target: caspase-6 p10

Epitope Species: human

Product Reactivity/Detection: p10 subunit and precursor of caspase-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for caspase-6 p10 Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin