caspase-3 Antibody (4i29): sc-56050
- caspase-3 Antibody (4i29) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against full length caspase-3 of human origin
- recommended for detection of full-length caspase-3 at Mr 32 kDa and 17 kDa subunit of 10 ng recombinant of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for caspase-3 Antibody (4i29) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (4i29) (see ordering information below).
QUICK LINKS
SEE ALSO...
caspase-3 Antibody (4i29) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect the full-length human caspase-3 protein, also known as apopain, SCA-1, Yama, and CPP32. caspase-3 monoclonal antibody (4i29) effectively recognizes caspase-3 across mouse, rat, and human species and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Caspase-3 is a pivotal aspartate-specific cysteine protease within the ICE subfamily, initially expressed as an inactive proenzyme that undergoes essential post-translational modifications, specifically proteolytic cleavage, to become activated. These post-translational modifications convert procaspase-3 into an active heterodimer composed of p17 and p11 subunits, enabling caspase-3 to execute its role in apoptosis by cleaving key substrates such as PARP, thereby orchestrating the orderly disassembly of cellular components. The structural integrity resulting from these modifications is essential for caspase-3′s enzymatic activity and interactions with other proteins in the apoptotic pathway, including the activation of caspase-6, -7, and -9, which amplifies apoptotic signals. Additionally, caspase-3 plays a role in regulating various cellular processes beyond apoptosis, such as the activation of SREBPs, which are crucial for lipid metabolism. The precise post-translational modifications of caspase-3 ensure proper localization and function within the cytoplasm, maintaining cellular homeostasis by eliminating damaged or unnecessary cells, a process vital for development, immune response, and cancer prevention. anti-caspase-3 antibody (4i29) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options for diverse research needs. caspase-3 (4i29) antibody is an indispensable tool for scientists investigating apoptosis and related cellular mechanisms, making caspase-3 (4i29) monoclonal antibody essential for research in fields such as cancer biology, immunology, and neurodegenerative diseases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-3 Antibody (4i29) | sc-56050 | 200 µg/ml | $316.00 | |||
caspase-3 Antibody (4i29): m-IgG Fc BP-HRP Bundle | sc-537091 | 200 µg Ab; 10 µg BP | $354.00 | |||
caspase-3 Antibody (4i29): m-IgGκ BP-HRP Bundle | sc-534278 | 200 µg Ab; 40 µg BP | $354.00 |