ZNF429 Activators
ZNF429 can function through various cellular pathways to modulate its activity. Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC), which can phosphorylate ZNF429, leading to changes in its ability to bind DNA and regulate gene expression. Similarly, Forskolin raises intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA can also phosphorylate ZNF429, potentially altering its activity. Ionomycin, by increasing intracellular calcium levels, can activate calmodulin-dependent kinase (CaMK), which may phosphorylate ZNF429 and affect its function. Retinoic acid operates through its nuclear receptors and may influence ZNF429 by promoting chromatin remodeling at gene promoters where ZNF429 can bind. The Epidermal Growth Factor (EGF) stimulates its receptor leading to signaling cascades, such as the Ras/MAPK and PI3K/Akt pathways, which can have downstream effects on ZNF429 phosphorylation and activity.
5-Azacytidine and histone deacetylase inhibitors such as Trichostatin A and Sodium butyrate can alter the chromatin state. 5-Azacytidine reduces DNA methylation, which can increase the binding of ZNF429 to its target DNA sequences. Trichostatin A and Sodium butyrate can cause chromatin to become more open, potentially improving the accessibility of ZNF429 to DNA. Thapsigargin, by inhibiting the SERCA pump, leads to increased cytosolic calcium levels, which can activate calcium-dependent kinases that may phosphorylate ZNF429. Zinc sulfate is critical for the proper structure and function of zinc finger proteins like ZNF429, ensuring that its DNA-binding domains are correctly configured. Dibutyryl-cAMP, a cAMP analog, activates PKA, which may phosphorylate and activate ZNF429. Lastly, Lithium chloride inhibits GSK-3, possibly affecting Wnt signaling and indirectly influencing the activity of ZNF429. Each chemical, through its unique mechanism, can modulate the activity state of ZNF429, impacting its role in regulating gene expression.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a known activator of protein kinase C (PKC). ZNF429, as a zinc finger protein, may have domains that are phosphorylated by PKC, leading to activation of its DNA-binding or transcription regulatory activity. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which could activate calmodulin-dependent kinase (CaMK). CaMK can phosphorylate proteins and potentially lead to the activation of ZNF429 if it has relevant phosphorylation sites. | ||||||
Retinoic Acid, all trans | 302-79-4 | sc-200898 sc-200898A sc-200898B sc-200898C | 500 mg 5 g 10 g 100 g | $66.00 $325.00 $587.00 $1018.00 | 28 | |
Retinoic acid acts through its nuclear receptors which can lead to chromatin remodeling and potentially enhance the DNA-binding activity of ZNF429 if it interacts with retinoic acid receptor-regulated gene promoters. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine inhibits DNA methyltransferases, leading to hypomethylation of DNA. This may enhance the binding of ZNF429 to its target DNA sequences if DNA methylation ordinarily inhibits its DNA-binding activity. | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $152.00 $479.00 $632.00 $1223.00 $2132.00 | 33 | |
Trichostatin A is a histone deacetylase inhibitor, which can cause chromatin to become more accessible. This could enhance the DNA-binding activity of ZNF429 if it targets areas of chromatin that are normally less accessible due to histone deacetylation. | ||||||
Sodium Butyrate | 156-54-7 | sc-202341 sc-202341B sc-202341A sc-202341C | 250 mg 5 g 25 g 500 g | $31.00 $47.00 $84.00 $222.00 | 19 | |
Sodium butyrate, another histone deacetylase inhibitor, similarly to Trichostatin A, could potentially increase the accessibility of the chromatin for ZNF429, enhancing its ability to bind DNA and activate transcription. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to an increase in cytosolic calcium levels, potentially activating calcium-dependent kinases that could phosphorylate and activate ZNF429. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc is a co-factor for many zinc finger proteins. Zinc sulfate supplementation could enhance the DNA-binding ability of ZNF429 by ensuring proper folding and structure of its zinc finger domains. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a membrane-permeable cAMP analog that activates PKA. PKA can phosphorylate substrates and may activate ZNF429 by phosphorylation. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium chloride inhibits GSK-3, which may lead to activation of Wnt signaling. Activation of Wnt target genes could include genes that encode proteins that interact with ZNF429, leading to its activation. | ||||||