ZFP869 Inhibitors
Chemical inhibitors of ZFP869 can modulate its activity through various biochemical pathways. Phorbol 12-myristate 13-acetate (PMA) and Bryostatin 1 are both activators of Protein Kinase C (PKC), which can phosphorylate ZFP869, enhancing its DNA-binding activity or promoting its interaction with other transcriptional coactivators. Similarly, Ionomycin, by increasing intracellular calcium levels, can activate calcium-dependent kinases such as PKC, which can also result in the phosphorylation of ZFP869. In contrast, Okadaic Acid and Calyculin A inhibit protein phosphatases like PP1 and PP2A, preventing the dephosphorylation of ZFP869 and maintaining it in an active phosphorylated state. This inhibition can keep ZFP869 in a state that allows it to continuously act as a transcription factor without the regulatory check that dephosphorylation by phosphatases would provide.
Forskolin and related compounds such as 8-Br-cAMP and Dibutyryl cAMP increase cAMP levels in cells, which in turn activate Protein Kinase A (PKA). PKA can then phosphorylate ZFP869, potentially increasing its transcription factor activity by promoting its binding to DNA or recruitment of cofactors. PDBu, a PKC activator that mimics diacylglycerol (DAG), can lead to the phosphorylation of ZFP869, enhancing its transcriptional activity by promoting its nuclear localization or DNA-binding ability. Additionally, Anisomycin, which is a protein synthesis inhibitor, can activate stress-activated protein kinases (SAPKs) that can phosphorylate ZFP869, possibly enhancing its function by increasing its stability or DNA-binding activity. Lastly, Fusicoccin can potentiate ZFP869's activity by stabilizing the interaction between 14-3-3 proteins and phosphorylated ZFP869, which could enhance its function as a transcription factor.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates Protein Kinase C (PKC) which is known to phosphorylate a myriad of proteins. ZFP869 activation can result from PKC-mediated phosphorylation that enhances its DNA-binding activity or promotes its interaction with other coactivators. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, leading to an increase in cAMP levels and subsequent activation of PKA. PKA can phosphorylate ZFP869, which may enhance its transcription factor activity by promoting its binding to DNA or recruitment of cofactors. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium-dependent kinases such as PKC. This activation can result in the phosphorylation of ZFP869, leading to an increase in its transcription factor activity. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid inhibits protein phosphatases PP1 and PP2A. Inhibition of these phosphatases prevents dephosphorylation of ZFP869, maintaining it in an activated phosphorylated state, which may enhance its function as a transcription factor. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Br-cAMP is a cAMP analog that activates PKA. Upon activation, PKA may phosphorylate ZFP869, potentially increasing its activity as a transcription factor by facilitating its binding to DNA or interaction with other proteins. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $240.00 | 9 | |
Bryostatin 1 is a PKC activator that can promote the phosphorylation of ZFP869, potentially enhancing its activity as a transcription factor by increasing its DNA-binding activity or interaction with transcriptional machinery. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs). These kinases can phosphorylate ZFP869, potentially enhancing its function by increasing its stability or DNA-binding activity. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the SERCA pump, leading to increased cytosolic calcium levels, which can activate PKC. Activated PKC might phosphorylate ZFP869, potentially increasing its transcriptional activity. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A inhibits protein phosphatases such as PP1 and PP2A. This inhibition can lead to the sustained phosphorylation of ZFP869, potentially keeping it in an activated state conducive to its role as a transcription factor. | ||||||
Phorbol-12,13-dibutyrate | 37558-16-0 | sc-202285 | 1 mg | $122.00 | 3 | |
PDBu is a PKC activator that can mimic diacylglycerol (DAG). It can lead to the phosphorylation of ZFP869, which might enhance its transcriptional activity by promoting its nuclear localization or DNA-binding ability. | ||||||