ZFP719 Activators
Chemical activators of ZFP719 can modulate its activity through various biochemical pathways, primarily by altering its phosphorylation status. Phorbol 12-myristate 13-acetate (PMA) is known to directly activate protein kinase C (PKC), which can phosphorylate ZFP719, promoting its DNA-binding capacity and altering its role in gene expression regulation. Similarly, Ionomycin functions as an ionophore that increases intracellular calcium levels, which can activate calcium-dependent kinases. These kinases, in turn, can phosphorylate ZFP719, potentially enhancing its function in cellular processes. Forskolin, by stimulating adenylate cyclase, raises cAMP levels within the cell, which activates protein kinase A (PKA). PKA can phosphorylate ZFP719, possibly augmenting its activity related to transcriptional regulation.
Additionally, Okadaic Acid and Calyculin A, both inhibitors of protein phosphatases 1 and 2A, lead to an increased phosphorylated state of cellular proteins. This could result in the sustained activation of ZFP719 by preventing its dephosphorylation. Thapsigargin, by inhibiting the SERCA pump, leads to a rise in cytosolic calcium levels and subsequent activation of calcium-dependent kinases that can phosphorylate ZFP719. 8-Br-cAMP and Dibutyryl-cAMP (db-cAMP), both cAMP analogs, diffuse into cells and activate PKA, which then can phosphorylate ZFP719, thus promoting its function in gene expression regulation. Anisomycin, which activates MAP kinase pathways, can lead to the activation of kinases that may phosphorylate ZFP719. Cantharidin, another inhibitor of protein phosphatases, can increase the phosphorylation state of ZFP719, enhancing its activity. Chelerythrine, a PKC inhibitor, may induce the activation of alternative kinases that can phosphorylate ZFP719. Together, these chemical activators can modulate ZFP719 activity by influencing its phosphorylation, a key post-translational modification that regulates protein function.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA directly activates protein kinase C (PKC) which is known to phosphorylate a multitude of proteins. Given that ZFP719 is a zinc finger protein, PKC-mediated phosphorylation can lead to the activation of ZFP719 by altering its conformation or interaction with other molecules, thus promoting its DNA-binding capacity and function in gene expression regulation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin functions as an ionophore that selectively binds to calcium ions and transports them across cell membranes. The increase in intracellular calcium levels can activate calcium-dependent protein kinases, which could then phosphorylate ZFP719, leading to its activation in cellular processes such as DNA binding and transcriptional regulation. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylate cyclase, leading to an increase in cAMP levels within the cell. Elevated cAMP activates protein kinase A (PKA), and PKA can then phosphorylate ZFP719, which would enhance its DNA-binding activity and its role in transcriptional regulation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 and 2A. Inhibition of these phosphatases results in an increase in the phosphorylated state of proteins within the cell. This can lead to the activation of ZFP719 by maintaining its phosphorylation status, which is essential for its function in the regulation of gene expression. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Calyculin A, similar to Okadaic Acid, is an inhibitor of protein phosphatases, particularly PP1 and PP2A. By inhibiting these phosphatases, Calyculin A can cause an increase in phosphorylation levels of proteins, including ZFP719. This sustained phosphorylation can activate ZFP719, promoting its role in cellular processes like transcriptional regulation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to an increase in cytosolic calcium levels. The elevation of calcium can activate calcium-dependent kinases, which may phosphorylate and thereby activate ZFP719, allowing it to exert its function in regulating gene expression. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Br-cAMP is a cell-permeable cAMP analog that activates protein kinase A (PKA) without the need for receptor interaction. PKA activation can lead to the phosphorylation of ZFP719, which is expected to result in its activation and subsequent involvement in transcriptional regulation within the cell. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is known to activate MAP kinase pathways indirectly. Activation of these pathways can lead to the activation of downstream kinases that are capable of phosphorylating various substrates, including ZFP719, thereby potentially activating it and enabling its role in the regulation of gene expression. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is another cAMP analog that diffuses into cells and activates PKA. PKA can then phosphorylate ZFP719, which would lead to its functional activation, facilitating its role in the transcriptional regulation of genes. | ||||||
Cantharidin | 56-25-7 | sc-201321 sc-201321A | 25 mg 100 mg | $81.00 $260.00 | 6 | |
Cantharidin is an inhibitor of protein phosphatases 1 and 2A. By inhibiting these enzymes, there is an increase in the phosphorylation of proteins, which could include ZFP719. This increase in phosphorylation state is likely to activate ZFP719, enabling it to fulfill its function in cellular regulatory mechanisms. | ||||||