ZFP677 Inhibitors
Chemical inhibitors of ZFP677 act through various intracellular signaling mechanisms to modulate the activity of this protein. Bisindolylmaleimide I and Phorbol 12-myristate 13-acetate (PMA) are both activators of protein kinase C (PKC), which can phosphorylate ZFP677, leading to an elevation in its activity. This phosphorylation induces a conformational change or alters its interaction with DNA, enhancing ZFP677's functionality. Similarly, Forskolin, by increasing intracellular cAMP levels, indirectly stimulates protein kinase A (PKA), which can also target ZFP677 for phosphorylation, potentially increasing its activity. Another chemical, Dibutyryl-cyclic AMP (db-cAMP), a synthetic analog of cAMP, directly activates PKA, which can then phosphorylate and activate ZFP677.
In contrast, Okadaic Acid, by inhibiting protein phosphatases PP1 and PP2A, leads to an overall increase in the phosphorylation state of proteins within the cell, which includes ZFP677, thus promoting its activity by decreasing the rate of dephosphorylation. Cantharidin operates in a similar fashion by inhibiting serine/threonine phosphatases, which would otherwise act to deactivate ZFP677 by removing phosphate groups. On the other hand, Ionomycin works by increasing intracellular calcium levels, which can activate calcium-dependent kinases capable of phosphorylating ZFP677, thereby influencing its activity. 1,2-Dioctanoyl-sn-glycerol (DiC8), a synthetic analog of diacylglycerol (DAG), activates PKC as well, leading to the phosphorylation and potential activation of ZFP677. Furthermore, 6-Benzylaminopurine activates cyclin-dependent kinases (CDKs), which may target ZFP677 for phosphorylation, potentially affecting its activity state. Lastly, Anisomycin activates stress-activated protein kinases (SAPKs), such as JNK, which can phosphorylate and regulate ZFP677 as part of the cell's response to stress, which often involves the regulation of transcription factors through phosphorylation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $103.00 $237.00 | 36 | |
Bisindolylmaleimide I directly activates protein kinase C (PKC), which is known to phosphorylate serine and threonine residues on target proteins. Phosphorylation by PKC on ZFP677 can lead to an increase in its activity by changing its conformation or its interaction with DNA. | ||||||
Piceatannol | 10083-24-6 | sc-200610 sc-200610A sc-200610B | 1 mg 5 mg 25 mg | $50.00 $70.00 $195.00 | 11 | |
Piceatannol selectively inhibits Syk kinase, which can lead to the activation of alternate signaling pathways that may converge on the activation of ZFP677 through cross-talk, as Syk inhibition can lead to compensatory activation of related proteins. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing cAMP levels, which in turn activates PKA. PKA can phosphorylate ZFP677, resulting in an increase in its functional activity, as direct phosphorylation often activates transcription factors. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium-dependent kinases like calmodulin kinase. These kinases could phosphorylate ZFP677, leading to its functional activation as a result of changes in protein conformation or function. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid inhibits protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels of proteins due to reduced dephosphorylation. This inhibition can result in the activation of ZFP677 as phosphorylation often regulates transcription factor activity. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates PKC, and PKC-mediated phosphorylation of ZFP677 can lead to an increase in its transcriptional activity, as PKC typically phosphorylates proteins to regulate their function. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP, a cAMP analog, directly activates PKA, which may phosphorylate and thus activate ZFP677, as PKA-mediated phosphorylation is known to regulate the activity of various proteins, including transcription factors. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $46.00 $249.00 | 2 | |
DiC8 is a synthetic diacylglycerol (DAG) analog that activates PKC. PKC activation can result in the phosphorylation and subsequent activation of ZFP677, likely through direct interaction and modification of the protein. | ||||||
6-Benzylaminopurine | 1214-39-7 | sc-202428 sc-202428A | 1 g 5 g | $20.00 $51.00 | ||
6-Benzylaminopurine, a cytokinin, can activate cyclin-dependent kinases (CDKs), which may phosphorylate ZFP677, leading to its activation through changes in its phosphorylation state that affect its functional activity. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates stress-activated protein kinases (SAPKs), such as JNK, which may then phosphorylate and activate ZFP677 as part of the cellular stress response, whereby transcription factors are often regulated through phosphorylation. | ||||||