WDR27 Activators
Chemical activators of WDR27 can initiate various cellular signaling pathways, leading to its activation through phosphorylation. Forskolin, by raising intracellular cAMP levels, indirectly stimulates protein kinase A (PKA), which may then phosphorylate WDR27, altering its activity state. Similarly, dibutyryl-cAMP, a cAMP analog, diffuses into cells and mimics Forskolin's effects by also activating PKA, potentially resulting in the phosphorylation of WDR27. Phorbol 12-myristate 13-acetate (PMA) functions through a different mechanism, activating protein kinase C (PKC), which phosphorylates serine and threonine residues on a plethora of target proteins, possibly including WDR27. This post-translational modification can modulate WDR27's function within the cell. On another front, ionomycin increases intracellular calcium concentrations, which may activate calcium/calmodulin-dependent protein kinases (CaMKs). These kinases can phosphorylate various proteins within calcium-signaling pathways, potentially impacting WDR27's activation state.
Further, Calyculin A and Okadaic acid, both inhibitors of protein phosphatases PP1 and PP2A, prevent the dephosphorylation of proteins, which could lead to sustained phosphorylation and consequent activation of WDR27 if it is normally regulated by these phosphatases. Epigallocatechin gallate (EGCG) can activate AMP-activated protein kinase (AMPK), which may phosphorylate WDR27 directly or indirectly by targeting associated regulatory proteins. Similarly, Spermine NONOate releases nitric oxide that can increase cGMP levels, activating protein kinase G (PKG) that may phosphorylate and activate WDR27. Anisomycin, a stress-activated kinase activator, potentially involves WDR27 in the cellular response to stress by leading to its phosphorylation through activated Jun N-terminal kinase (JNK) pathways. Zaprinast inhibits phosphodiesterase 5, thereby increasing cGMP levels, which in turn may enhance PKG activity and lead to the phosphorylation of WDR27. Lastly, A23187 (Calcimycin), a calcium ionophore, raises cytosolic calcium levels, potentially leading to the activation of WDR27 through calcium-dependent protein kinases.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate serine and threonine residues on various proteins. PKC-mediated phosphorylation can lead to the activation of WDR27 by altering its conformation or interaction with other cellular components within signaling cascades where WDR27 is a participant. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium levels, which in turn can activate calcium/calmodulin-dependent protein kinases (CaMKs). The activation of these kinases can lead to the phosphorylation of substrates involved in the same signaling pathways as WDR27, potentially resulting in the functional activation of WDR27 through a cascade of phosphorylation events. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable analog of cAMP that activates PKA. PKA then phosphorylates target proteins involved in the cAMP signaling pathway, which may include WDR27, leading to its activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatase 1 (PP1) and 2A (PP2A). By inhibiting these phosphatases, Calyculin A prevents the dephosphorylation of proteins, potentially resulting in the sustained activation of WDR27 if it is normally regulated by PP1 or PP2A-mediated dephosphorylation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid selectively inhibits protein phosphatases PP1 and PP2A, leading to an increase in the phosphorylation state of proteins within the cell. This inhibition can cause the accumulation of phosphorylated WDR27, maintaining its activation if PP1 or PP2A normally regulate its activity via dephosphorylation. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG has been shown to activate AMP-activated protein kinase (AMPK). Activation of AMPK can initiate a phosphorylation cascade that may include WDR27, depending on its role in the cellular context, thereby promoting the activation of WDR27 through direct phosphorylation or by phosphorylation of associated regulatory proteins. | ||||||
Spermine NONOate | 136587-13-8 | sc-202816 sc-202816A | 5 mg 25 mg | $53.00 $196.00 | 5 | |
Spermine NONOate donates nitric oxide which can activate guanylyl cyclase, increasing cyclic GMP (cGMP) levels. Elevated cGMP can activate PKG, which may phosphorylate and thus activate WDR27 if it is a substrate for PKG or involved in pathways regulated by PKG. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin activates stress-activated protein kinases (SAPKs), such as JNK. The activation of these kinases can lead to the phosphorylation of proteins that are part of stress-related signaling pathways, including potentially WDR27, resulting in its activation. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $105.00 $250.00 | 8 | |
Zaprinast inhibits phosphodiesterase 5 (PDE5), leading to an increase in cGMP levels within the cell. This increase can enhance the activity of PKG, which in turn can phosphorylate and activate proteins involved in the cGMP signaling pathway, potentially including WDR27. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 acts as a calcium ionophore, increasing intracellular calcium concentration. This elevation in calcium can activate calcium-dependent enzymes and signaling pathways that may result in the activation of WDR27, assuming it is part of or regulated by calcium-dependent mechanisms. | ||||||