TFIIIC35 Activators
Chemical activators of TFIIIC35 can initiate a cascade of biochemical events leading to the protein's activation. Zinc chloride and magnesium chloride engage with TFIIIC35 by stabilizing its structure and the transcription complex it forms part of, ensuring proper folding and conformation. This stabilization is crucial as it aids in the maintenance of the protein's functionality during the initiation of RNA polymerase III transcription. Concurrently, sodium fluoride's inhibitory effect on serine/threonine phosphatases results in the upregulation of TFIIIC35's phosphorylation state, a modification that is indispensable for its activation. Likewise, potassium chloride contributes to this process by optimizing the ionic environment, which is essential for the structural integrity of TFIIIC35 and its interaction with other molecules involved in transcription.
Further activation of TFIIIC35 arises through the action of forskolin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). Forskolin raises cAMP levels, indirectly causing the activation of protein kinase A, which then phosphorylates TFIIIC35. Ionomycin, by increasing intracellular calcium, can activate calcium-dependent kinases that may target TFIIIC35 for phosphorylation. PMA's role in the activation of protein kinase C leads to similar phosphorylative activation of TFIIIC35. AICAR triggers the activation of AMP-activated protein kinase, another enzyme that can phosphorylate and activate TFIIIC35. Conversely, calyculin A, cantharidin, and okadaic acid maintain TFIIIC35 in an active state by inhibiting protein phosphatases, thus preventing the dephosphorylation and subsequent inactivation of the protein. Lastly, anisomycin prompts the activation of stress-activated protein kinases which, in turn, can phosphorylate and activate TFIIIC35, enhancing its role in transcription initiation by RNA polymerase III. Each chemical, through its unique mechanism, ensures that TFIIIC35 is phosphorylated and consequently active, facilitating its critical role in gene expression.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc chloride can activate TFIIIC35 by serving as a structural cofactor, enhancing the protein's ability to correctly fold and maintain the conformation necessary for its function in initiation of RNA polymerase III transcription. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $28.00 $35.00 $48.00 $125.00 | 2 | |
Magnesium chloride activates TFIIIC35 by stabilizing the RNA polymerase III transcription complex, of which TFIIIC35 is a critical component, thus facilitating its transcriptional activity. | ||||||
Sodium Fluoride | 7681-49-4 | sc-24988A sc-24988 sc-24988B | 5 g 100 g 500 g | $40.00 $46.00 $100.00 | 26 | |
Sodium fluoride activates TFIIIC35 through the inhibition of serine/threonine phosphatases, leading to an increased phosphorylation state of the protein, which is essential for its activation and function in transcription complex assembly. | ||||||
Potassium Chloride | 7447-40-7 | sc-203207 sc-203207A sc-203207B sc-203207C | 500 g 2 kg 5 kg 10 kg | $55.00 $155.00 $285.00 $455.00 | 5 | |
Potassium chloride can activate TFIIIC35 by affecting the ionic environment, which is essential for the maintenance of the protein's structure and its interaction with other transcription factors and RNA polymerase III. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin activates TFIIIC35 by increasing intracellular calcium levels, which can activate calcium-dependent kinases. These kinases may phosphorylate and activate TFIIIC35, thereby promoting its role in transcription complex formation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates TFIIIC35 by activating protein kinase C (PKC), which can phosphorylate TFIIIC35, resulting in its activation and the promotion of its function in the assembly of the RNA polymerase III transcription complex. | ||||||
AICAR | 2627-69-2 | sc-200659 sc-200659A sc-200659B | 50 mg 250 mg 1 g | $65.00 $280.00 $400.00 | 48 | |
AICAR activates TFIIIC35 through the activation of AMP-activated protein kinase (AMPK), which can phosphorylate and hence activate TFIIIC35, enhancing its role in transcription initiation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A activates TFIIIC35 by inhibiting protein phosphatases that dephosphorylate and deactivate TFIIIC35, thereby maintaining the protein in a phosphorylated and active state for RNA polymerase III transcription initiation. | ||||||
Cantharidin | 56-25-7 | sc-201321 sc-201321A | 25 mg 100 mg | $89.00 $279.00 | 6 | |
Cantharidin activates TFIIIC35 by inhibiting serine/threonine phosphatases, leading to a sustained phosphorylated state of TFIIIC35 which is essential for its activation and function in the transcription process. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid activates TFIIIC35 by selectively inhibiting protein phosphatases 1 and 2A, leading to increased phosphorylation and activation of TFIIIC35, which is necessary for its role in the initiation of transcription by RNA polymerase III. | ||||||