SSXB5 Activators
Chemical activators of SSXB5 can initiate a variety of intracellular signaling pathways that result in the phosphorylation and consequent activation of this protein. Ionomycin functions by transporting calcium ions into cells, raising the intracellular calcium levels which in turn activate calcium-dependent kinases. These kinases then phosphorylate SSXB5, leading to its activation as part of the calcium signaling cascade. Thapsigargin also elevates cytosolic calcium by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), thus triggering a similar cascade of calcium-dependent activation of SSXB5. In a somewhat parallel mechanism, Forskolin increases intracellular cAMP, which activates Protein Kinase A (PKA). PKA then phosphorylates various substrates within its signaling pathway, potentially including SSXB5 if it is a target for this kinase. Dibutyryl cAMP, a permeable cAMP analog, also activates PKA, suggesting another route through which SSXB5 can be phosphorylated and activated.
Further, activators such as Phorbol 12-myristate 13-acetate (PMA) can activate Protein Kinase C (PKC), which may phosphorylate SSXB5 if it is a PKC substrate, leading to its activation. Bisindolylmaleimide I, as a PKC inhibitor, could maintain SSXB5 in its phosphorylated state by preventing PKC from dephosphorylating it. Additionally, Epidermal Growth Factor (EGF) engages its receptor EGFR, initiating a kinase cascade that can result in the phosphorylation of SSXB5. Inhibition of protein phosphatases with compounds like Okadaic Acid and Calyculin A leads to sustained phosphorylation and, therefore, activation of proteins like SSXB5. Sodium Orthovanadate, by inhibiting protein tyrosine phosphatases, also prevents dephosphorylation, potentially contributing to SSXB5 activation. Lastly, anisomycin stimulates stress-activated protein kinases (SAPKs) which can phosphorylate SSXB5 as part of the stress response signaling pathway.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin acts as a calcium ionophore, selectively transporting calcium ions across cell membranes. This elevated intracellular calcium concentration activates calcium-dependent kinases which are known to phosphorylate numerous proteins, including SSXB5. The phosphorylation directly leads to the activation of SSXB5 as part of the calcium signaling pathway. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA acts as a diacylglycerol analog, activating Protein Kinase C (PKC). PKC phosphorylates target proteins, and assuming SSXB5 is a substrate for PKC, this would result in the direct activation of SSXB5 through phosphorylation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leading to increased cytosolic calcium levels. This triggers the activation of calcium-dependent proteins and kinases which can phosphorylate and activate SSXB5 as part of the calcium signaling pathway. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl cAMP is a cell-permeable cAMP analog that activates Protein Kinase A (PKA) by mimicking cAMP, leading to the phosphorylation and activation of proteins within the PKA signaling pathway, potentially including SSXB5. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 and 2A, which prevents dephosphorylation of proteins. This indirect activation maintains proteins like SSXB5 in a phosphorylated state, thereby sustaining their activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to Okadaic Acid, Calyculin A is an inhibitor of protein phosphatases 1 and 2A. By inhibiting these phosphatases, it keeps proteins such as SSXB5 phosphorylated and thus activated. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin activates stress-activated protein kinases (SAPKs), which can lead to the phosphorylation and subsequent activation of substrates involved in the stress response pathway, potentially including SSXB5. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
Sodium Orthovanadate is an inhibitor of protein tyrosine phosphatases. It prevents the dephosphorylation of tyrosine residues on proteins, potentially leading to the activation of proteins such as SSXB5 through sustained phosphorylation. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is a specific inhibitor of Protein Kinase C (PKC). It is used here as a tool to indirectly infer activation of SSXB5 through PKC pathways, as it would maintain SSXB5 in an active state by preventing dephosphorylation when PKC is inhibited. | ||||||
H-89 dihydrochloride | 130964-39-5 | sc-3537 sc-3537A | 1 mg 10 mg | $94.00 $186.00 | 71 | |
H-89 is a potent inhibitor of Protein Kinase A (PKA). By inhibiting PKA, it can be used to deduce the activation state of substrates in the PKA pathway, such as SSXB5, by preserving the phosphorylated and active form of SSXB5 when PKA activity is reduced. | ||||||