Sec1 Activators
Sec1 Activators are a collection of chemical compounds that serve to indirectly enhance the activity of Sec1 through modulation of various signaling pathways, thereby facilitating its role in vesicle trafficking within the cell. Forskolin, for example, increases intracellular cAMP levels, which in turn activates PKA. This activation can lead to phosphorylation events that can enhance the interaction of Sec1 with related proteins, bolstering its role in vesicle trafficking. Similarly, the use of PMA, a PKC activator, can indirectly promote Sec1's activity by potentiating the SNARE complex, essential for vesicle fusion, a process to which Sec1 is central. Ionomycin and A23187, both calcium ionophores, amplify intracellular calcium levels, which can trigger signaling that favors Sec1's role in vesicle docking and fusion. The cAMP analogs 8-Br-cAMP, 6-Bnz-cAMP, and db-cAMP all uniformly activate PKA, potentially enhancing Sec1's role by promoting phosphorylation within the vesicle trafficking pathway.
Further influencing Sec1 activity, compounds like Epigallocatechin gallate (EGCG) and Brefeldin A affect various kinases and cellular structures, respectively, leading to changes in the vesicle trafficking network that can indirectly enhance Sec1's function. Okadaic acid, a phosphatase inhibitor, maintains phosphorylation states of proteins within the vesicle trafficking pathway, potentially heightening the functional role of Sec1. Thapsigargin, by inhibiting the SERCA pump, results in raised cytosolic calcium levels, which may activate calcium-dependent signals that synergize with Sec1's activity. Cilostamide works by inhibiting PDE3, leading to increased cAMP levels and thereby facilitating PKA activation, which may indirectly enhance Sec1's function in vesicle trafficking. Collectively, these chemicals act through distinct but converging pathways that contribute to the activation of Sec1, ensuring its role in vesicle trafficking is maintained or enhanced without directly increasing the expression or direct activation of the protein.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP activates protein kinase A (PKA), which can phosphorylate various targets, potentially including Sec1 or its associated binding partners, thereby enhancing Sec1's role in vesicle trafficking. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is a catechin found in green tea with kinase inhibition properties. By inhibiting certain kinases, EGCG could reduce competitive signaling, thus indirectly enhancing Sec1's function in membrane fusion and neurotransmitter release. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $162.00 $316.00 $559.00 $889.00 $1693.00 | 7 | |
This lipid signaling molecule activates sphingosine-1-phosphate receptors, which can lead to downstream effects on cellular signaling pathways, such as those regulating vesicle trafficking, potentially enhancing Sec1's activity in exocytosis. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is a potent activator of protein kinase C (PKC) which can phosphorylate substrates that may interact with Sec1, leading to enhanced vesicle trafficking and secretion processes where Sec1 is involved. | ||||||
Ionomycin, free acid | 56092-81-0 | sc-263405 sc-263405A | 1 mg 5 mg | $94.00 $259.00 | 2 | |
Ionomycin is a calcium ionophore, raising intracellular calcium levels, which may activate calcium-dependent kinases and phosphatases, indirectly enhancing Sec1's role in vesicular transport and neurotransmitter release. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
Brefeldin A inhibits ADP-ribosylation factor (ARF), a small GTPase involved in vesicle formation. Inhibition of ARF could enhance Sec1's activity indirectly by modulating vesicle trafficking pathways. | ||||||
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $66.00 $219.00 $417.00 | 97 | |
Wortmannin is a specific inhibitor of PI3-kinase, which can lead to alterations in vesicular trafficking and fusion events, potentially enhancing Sec1-mediated processes by affecting the membrane dynamics. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is another PI3-kinase inhibitor that may indirectly enhance Sec1's activity by altering vesicle formation and trafficking, a process in which Sec1 is implicated. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $106.00 $166.00 $289.00 $550.00 $819.00 | 2 | |
This cAMP analog specifically activates PKA, which can phosphorylate proteins involved in vesicle trafficking, potentially enhancing the function of Sec1 in this process. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels of proteins that may interact with or regulate Sec1, thus enhancing its activity. | ||||||