POU2F3 Activators
POU2F3 activators span a collection of chemical compounds that influence a variety of signaling pathways and cellular processes, ultimately leading to the upregulation of POU2F3's functional activity. Forskolin, isoproterenol, and 8-Bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) each play a role in increasing intracellular cAMP levels, leading to the activation of PKA. This kinase phosphorylates substrates within the cellular milieu that may interact with POU2F3, enhancing its transcriptional activity by facilitating its DNA binding and interaction with coactivators. Similarly, IBMX maintains elevated cAMP levels by inhibiting phosphodiesterases, leading to sustained PKA activity and potentially increasing POU2F3's transcriptional regulation. Phorbol 12-myristate 13-acetate (PMA) activates PKC, which in turn might engage in cross-talk with pathways that modulate POU2F3's activity, possibly by altering its phosphorylation status or its protein-protein interactions that are essential for its regulatory functions.
The transcription factor's activity is further influenced by compounds that modulate cellular differentiation and chromatin remodeling. Retinoic acid, by activating its receptors, may co-regulate genes with POU2F3, particularly in differentiation pathways where POU2F3 is critical. Ionomycin and A23187, both of which increase intracellular calcium, can activate calcium-sensitive kinases, potentially affecting POU2F3 activity by altering its phosphorylation state or modifying its interaction with transcriptional machinery. Sodium butyrate and Trichostatin A, as histone deacetylase inhibitors, can enhance POU2F3's access to chromatin, thereby facilitating its transcriptional activity in sensory functions. Epigallocatechin gallate (EGCG), through its kinase inhibitory effects, might reduce competitive signaling, indirectly leading to an upregulated POU2F3 function. Collectively, these activators operate through distinct biochemical mechanisms to enhance POU2F3's role as a transcription factor, crucial for gene expression in various cellular contexts, particularly those involving sensory receptor gene regulation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, increasing cyclic AMP (cAMP) levels in cells. Elevated cAMP activates protein kinase A (PKA), which can phosphorylate transcription factors including POU2F3, enhancing its transcriptional activity on target genes associated with olfactory and possibly other sensory functions. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which then phosphorylates target proteins. This activation can lead to changes in transcription factors and subsequently enhance the activity of POU2F3 by promoting its binding to DNA and transcription of downstream genes involved in cell differentiation and growth. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that raises intracellular cAMP levels, similar to Forskolin, leading to activation of PKA. PKA can then enhance POU2F3's role in transcriptional regulation of genes involved in cellular response to hormones and neurotransmitters. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, enzymes that break down cAMP. By preventing cAMP degradation, IBMX indirectly increases PKA activity, which in turn may enhance the transcriptional activity of POU2F3. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs) like JNK. Activation of JNK pathway can result in the phosphorylation of transcription factors and enhance POU2F3-mediated gene expression linked with stress response. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $106.00 $166.00 $289.00 $550.00 $819.00 | 2 | |
8-Br-cAMP (8-Bromoadenosine 3',5'-cyclic monophosphate) is a cAMP analog that activates PKA. Upon PKA activation, POU2F3 transcriptional activity is enhanced due to phosphorylation, affecting genes that are crucial for sensory neuron function and development. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is known to inhibit several protein kinases, potentially altering the phosphorylation state of various transcription factors. This change in the cellular phosphorylation landscape can indirectly enhance POU2F3 activity by affecting its interaction with other proteins and its binding to DNA. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calcium-dependent protein kinases. These kinases may phosphorylate POU2F3 or its co-factors, enhancing its transcriptional activity on genes involved in development and sensory processes. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $75.00 $212.00 | 18 | |
Rolipram is a selective inhibitor of phosphodiesterase 4 (PDE4), leading to an increase in cAMP levels. Elevated cAMP can enhance POU2F3 activity by PKA-mediated phosphorylation, influencing genes important for neurogenesis and sensory functions. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A-23187 is a calcium ionophore that raises intracellular calcium levels, activating calcium/calmodulin-dependent protein kinases (CaMKs). Activation of CaMKs could potentially enhance POU2F3's activity by promoting its phosphorylation and its transcriptional regulation of genes linked with sensory perception. | ||||||