POLR2K Inhibitors
POLR2K inhibitors target a subunit of RNA polymerase II (POLR2K), an essential enzyme involved in the transcription process in eukaryotic cells. RNA polymerase II is responsible for synthesizing messenger RNA (mRNA) from a DNA template, a critical step in gene expression. POLR2K, specifically, is one of the smaller subunits of this enzyme, playing a key structural and functional role. Inhibitors of POLR2K affect the activity of RNA polymerase II by interacting with this subunit, leading to alterations in transcription regulation. These inhibitors may disrupt the assembly, stability, or interaction of RNA polymerase II with DNA or other transcriptional machinery components. As a result, POLR2K inhibition can impede the initiation or elongation phases of transcription, reducing mRNA synthesis and, consequently, influencing the expression of various genes.
The specificity of POLR2K inhibitors lies in their ability to selectively interfere with this particular subunit without necessarily disrupting the function of other subunits or types of RNA polymerases. This precision allows for fine-tuned modulation of gene expression, offering a valuable tool for studying the transcriptional dynamics in various cellular processes. POLR2K, being a part of the highly conserved RNA polymerase II complex, suggests that inhibitors targeting this subunit must be intricately designed to maintain specificity while avoiding broader effects on general transcription. Such chemical inhibitors are essential for understanding the nuances of transcriptional regulation at the molecular level, providing insight into the role of specific subunits within the polymerase and their contributions to overall gene expression and cellular homeostasis.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Fluorouracil | 51-21-8 | sc-29060 sc-29060A | 1 g 5 g | $37.00 $152.00 | 11 | |
By impeding the enzymatic action of thymidylate synthase, Fluorouracil could lead to a depletion of thymidine triphosphate and thus compromise DNA synthesis, resulting in the downregulation of POLR2K transcription. | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $176.00 $426.00 | 43 | |
Doxorubicin's intercalation into DNA strands and inhibition of topoisomerase II could cause conformational changes that decrease the initiation and elongation phases of POLR2K gene transcription. | ||||||
Mithramycin A | 18378-89-7 | sc-200909 | 1 mg | $55.00 | 6 | |
Mithramycin A binds selectively to guanine-cytosine-rich DNA sequences, potentially repressing the promoter activity of the POLR2K gene and thereby decreasing its transcription. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
Cisplatin forms intrastrand DNA crosslinks that may obstruct the binding and progression of RNA polymerase at the POLR2K gene locus, leading to reduced mRNA synthesis. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Etoposide's inhibition of topoisomerase II could result in DNA strand breaks, potentially hindering the transcriptional machinery from effectively transcribing the POLR2K gene. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D binds to the DNA template at the transcription initiation complex, preventing the elongation of POLR2K mRNA by physically blocking RNA polymerase movement. | ||||||
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $41.00 $74.00 $221.00 $247.00 $738.00 $1220.00 | 39 | |
Taxol's stabilization of microtubules can disrupt normal cell cycle progression, which may indirectly lead to a decrease in POLR2K expression by altering transcription factor availability. | ||||||
Methotrexate | 59-05-2 | sc-3507 sc-3507A | 100 mg 500 mg | $94.00 $213.00 | 33 | |
Methotrexate's inhibition of folate metabolism enzymes could result in a reduced supply of nucleotides, causing a decrease in POLR2K gene transcription. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $78.00 $260.00 | 18 | |
By inhibiting ribonucleotide reductase, Hydroxyurea may lead to a nucleotide pool imbalance, specifically decreasing the availability of deoxyribonucleotides necessary for POLR2K gene replication and transcription. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $69.00 | 2 | |
Chloroquine's ability to intercalate into DNA could disrupt the replication and transcription processes, which may lead to a decrease in the expression of genes, including POLR2K. | ||||||