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Methotrexate (CAS 59-05-2)

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Alternate Names:
Methotrexate is also known as Amethopterin.
Application:
Methotrexate is a cell cycle arresting agent that inhibits DHFR and reduces IL-1 production in splenic cells.
CAS Number:
59-05-2
Purity:
≥98%
Molecular Weight:
454.45
Molecular Formula:
C20H22N8O5
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Methotrexate is a cell cycle arrest agent with varying effects. Methotrexate arrests the cell cycle in late G1/S therefore inhibiting the synthesis of DNA, RNA, thymidylates, and proteins. In the previous cases the inhibition of enzymes involved in purine metabolism, leading to accumulation of adenosine, or the suppression of intercellular adhesion molecule expression by T cells as the main mechanism of action by Methotrexate. Methotrexate has been observed to inhibit dihydrofolate reductase (DHFR). Methotrexate may act to modulate inflammation by promoting adenosine release or inhibiting transmethylation reactions. In adherent splenic cells IL-1 production was decreased by Methotrexate.Additionally offered as Methotrexate hydrate (sc-215309).


Methotrexate (CAS 59-05-2) References

  1. A clinical approach to the use of methotrexate for sarcoidosis.  |  Baughman, RP. and Lower, EE. 1999. Thorax. 54: 742-6. PMID: 10413729
  2. Lymphomas in rheumatoid arthritis patients treated with methotrexate: a 3-year prospective study in France.  |  Mariette, X., et al. 2002. Blood. 99: 3909-15. PMID: 12010788
  3. Interaction of dihydrofolate reductase with methotrexate: ensemble and single-molecule kinetics.  |  Rajagopalan, PT., et al. 2002. Proc Natl Acad Sci U S A. 99: 13481-6. PMID: 12359872
  4. Clinical experience of methotrexate in Crohn's disease: response, safety and monitoring of treatment.  |  Parker, R., et al. 2010. Postgrad Med J. 86: 208-11. PMID: 20354043
  5. Acute treatment with methotrexate induces hippocampal dysfunction in a mouse model of breast cancer.  |  Yang, M., et al. 2012. Brain Res Bull. 89: 50-6. PMID: 22796103
  6. Effect of antiarthritic drugs on the enhanced interleukin-1 (IL-1) production by macrophages from adjuvant-induced arthritic (AA) rats.  |  DiMartino, MJ., et al. 1987. Agents Actions. 21: 348-50. PMID: 3500597
  7. The distribution and elimination of methotrexate in mouse blood and brain after concurrent administration of polysorbate 80.  |  Azmin, MN., et al. 1985. Cancer Chemother Pharmacol. 14: 238-42. PMID: 3995684
  8. The effect of methotrexate on levels of dUTP in animal cells.  |  Goulian, M., et al. 1980. J Biol Chem. 255: 10630-7. PMID: 7430142
  9. Polymerization of tubulin in apoptotic cells is not cell cycle dependent.  |  Pittman, SM., et al. 1994. Exp Cell Res. 215: 263-72. PMID: 7982469
  10. Antifolate analogs: mechanism of action, analytical methodology, and clinical efficacy.  |  Fleisher, M. 1993. Ther Drug Monit. 15: 521-6. PMID: 8122287
  11. Cell-cycle-stage specificity of the methotrexate block as resolved by X-ray-induced chromosome damage.  |  Richardson, VB., et al. 1994. Cytogenet Cell Genet. 66: 126-8. PMID: 8287684
  12. Characteristics of cancer cell death after exposure to cytotoxic drugs in vitro.  |  Huschtscha, LI., et al. 1996. Br J Cancer. 73: 54-60. PMID: 8554984
  13. New approaches to the treatment of advanced breast cancer.  |  Eckardt, JR. 1996. Anticancer Drugs. 7 Suppl 2: 41-6. PMID: 8862711
  14. The mechanism of action of methotrexate.  |  Cronstein, BN. 1997. Rheum Dis Clin North Am. 23: 739-55. PMID: 9361153
  15. Adult and two children with fetal methotrexate syndrome.  |  Bawle, EV., et al. 1998. Teratology. 57: 51-5. PMID: 9562676

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Methotrexate, 100 mg

sc-3507
100 mg
$92.00

Methotrexate, 500 mg

sc-3507A
500 mg
$209.00

What is the shipping temperature for this product?

Asked by: hawkeye11
This chemical, Methotrexate, can be shipped ambient.
Answered by: Chemical Support 1
Date published: 2017-04-07

Can this product be stored in solution?

Asked by: hawkeye11
This chemical, Methotrexate (CAS 59-05-2), can be stored in solution for one month at -20oC.
Answered by: Chemical Support 1
Date published: 2017-04-07
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Rated 3 out of 5 by from Great productThis product works very well in mouse tumor treatment.
Date published: 2018-10-15
Rated 4 out of 5 by from Great productMethotrexate | CAS 59-05-2 works as expected in mouse
Date published: 2018-10-03
Rated 5 out of 5 by from PMID 25837271 USED THIS PRODUCT.PMID 25837271 USED THIS PRODUCT. IT IS A VERY GOOD PRODUCT.
Date published: 2017-05-23
Rated 5 out of 5 by from A VERY GOOD PRODUCTI USED IT IN MY MANUSCRIPT. IT IS A VERY GOOD PRODUCT.
Date published: 2017-04-04
Rated 5 out of 5 by from GilGil, et. al. (PubMed ID 19155216) used methotrexate as a selection agent for cells that were successfully transfected with the pCDM8 vector containing cDNAs for CD11b and CD18. -SCBT Publication Review
Date published: 2015-04-27
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Methotrexate is rated 4.4 out of 5 by 5.
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