MICALCL Activators
MICALCL engage in various modes of action to modulate the protein's function by interacting with cellular components, primarily the cytoskeleton. Phalloidin and Jasplakinolide, for instance, target actin filaments directly. Phalloidin binds and stabilizes these filaments, resulting in enhanced actin polymerization. This enhances the interaction of MICALCL with the cytoskeleton, activating it due to increased actin binding. Similarly, Jasplakinolide promotes actin filament stabilization and nucleation, providing more substrate for MICALCL to bind to, thereby promoting its activation. Cytochalasin D and Latrunculin A, on the other hand, disrupt actin dynamics by inhibiting polymerization, which may initiate cellular mechanisms to activate MICALCL as part of the response to restore cytoskeletal integrity.
Other activators exert their effects indirectly by modulating signaling pathways and enzyme activities that impinge on the actin cytoskeleton. Epinephrine and Forskolin increase intracellular levels of cAMP, which then enhance actin filament dynamics, indirectly leading to the activation of MICALCL. Similarly, Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C, which phosphorylates substrates that interact with MICALCL, leading to its activation. On the other side of the phosphorylation equation, Calyculin A and Okadaic acid inhibit protein phosphatases, increasing the phosphorylation levels within the cell, which can trigger the activation of signaling pathways involving MICALCL. Ionomycin raises intracellular calcium concentration, which activates proteins that regulate MICALCL, while S-nitroso-N-acetylpenicillamine (SNAP) releases nitric oxide to activate proteins involved in actin dynamics, thereby potentially influencing MICALCL activity. Lastly, Paclitaxel stabilizes microtubules, thereby indirectly affecting the actin cytoskeleton and possibly leading to MICALCL activation to maintain cellular function amidst these changes.
Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Phalloidin | 17466-45-4 | sc-202763 | 1 mg | $229.00 | 33 | |
Phalloidin stabilizes actin filaments and can lead to increased actin polymerization. MICALCL, being an actin-associated protein, would have increased interaction with the cytoskeleton, leading to its functional activation through enhanced actin binding. | ||||||
Jasplakinolide | 102396-24-7 | sc-202191 sc-202191A | 50 µg 100 µg | $180.00 $299.00 | 59 | |
Jasplakinolide promotes actin filament stabilization and nucleation, enhancing the polymerization of actin. This stabilization could lead to the activation of MICALCL by providing more actin filament substrate for its associated activities. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $40.00 $102.00 $197.00 $1739.00 $16325.00 | ||
Epinephrine activates the β-adrenergic receptors leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP may enhance actin filament dynamics, potentially activating MICALCL through its association with the actin cytoskeleton. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly activates adenylyl cyclase, which increases levels of cAMP in cells, indirectly enhancing actin dynamics and potentially increasing the activity of MICALCL through its interaction with the actin cytoskeleton. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels within the cell. This can alter the phosphorylation state of proteins associated with MICALCL, leading to its activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC) which can phosphorylate substrates that may interact with MICALCL, thereby activating MICALCL through enhanced protein-protein interactions. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium concentration, which can activate proteins that interact with or regulate MICALCL, leading to its activation due to changes in calcium-sensitive signaling pathways. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits protein phosphatases, leading to increased phosphorylation of proteins. This can result in the activation of signaling pathways that include actin dynamics, potentially activating MICALCL. | ||||||
Latrunculin A, Latrunculia magnifica | 76343-93-6 | sc-202691 sc-202691B | 100 µg 500 µg | $260.00 $799.00 | 36 | |
Latrunculin A binds to actin monomers and prevents their polymerization. This disruption of actin dynamics can lead to compensatory mechanisms that activate MICALCL as the cell tries to restore cytoskeletal integrity. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $145.00 $442.00 | 64 | |
Cytochalasin D inhibits actin polymerization by binding to the barbed ends of actin filaments. This can lead to a cellular response that activates MICALCL to regulate actin filament assembly and maintain cellular functions. | ||||||