LRRC72 Activators
Chemical activators of LRRC72 can initiate a cascade of intracellular signaling pathways that lead to the protein's functional activation. Phorbol 12-myristate 13-acetate (PMA) is a potent activator of protein kinase C (PKC), which can phosphorylate a wide array of proteins. When PMA activates PKC, it may directly target LRRC72 or initiate a phosphorylation cascade that ultimately leads to the modification and activation of LRRC72. Similarly, Ionomycin acts by increasing the intracellular concentration of calcium, a secondary messenger that activates various calcium-sensitive signaling pathways. The elevated calcium levels can activate kinases which then can phosphorylate LRRC72, leading to its activation. Thapsigargin, which disrupts calcium homeostasis by inhibiting the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), would similarly increase cytosolic calcium and potentially result in LRRC72 activation through calcium-dependent kinases.
On a different pathway, Forskolin and Dibutyryl-cAMP (db-cAMP) both act to increase intracellular cAMP levels. Forskolin achieves this by stimulating adenylyl cyclase, while db-cAMP, as a cAMP analog, directly activates protein kinase A (PKA). PKA then can phosphorylate various proteins within the cell, possibly including LRRC72. Inhibitors of protein phosphatases like Okadaic Acid and Calyculin A prevent the dephosphorylation of proteins, which can result in a net increase in phosphorylation within the cell, potentially affecting LRRC72. Pervanadate, as a tyrosine phosphatase inhibitor, can lead to an increase in tyrosine phosphorylation, which, if relevant to LRRC72, would activate it. Anisomycin activates stress-activated protein kinases such as JNK, which can phosphorylate and activate LRRC72 if it is a substrate or part of the JNK pathway. Lastly, while KN-93 is an inhibitor of the Ca2+/calmodulin-dependent protein kinase II (CaMKII), its action can lead to the compensatory activation of alternative pathways that may include LRRC72, resulting in its activation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC) which is known to phosphorylate a wide range of target proteins. Since LRRC72 has leucine-rich repeat domains, which are often involved in protein-protein interactions, the activation of PKC could lead to the phosphorylation of LRRC72, resulting in its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin functions as a calcium ionophore, increasing intracellular calcium levels. Elevated intracellular calcium can activate calcium-sensitive signaling proteins, which could include kinases that phosphorylate LRRC72, resulting in its functional activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium levels. This increase can activate calcium-dependent kinases that may phosphorylate LRRC72, thereby functionally activating the protein. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin stimulates adenylyl cyclase, increasing intracellular cAMP levels, which in turn activates protein kinase A (PKA). PKA can phosphorylate various proteins, and if LRRC72 is a substrate for PKA or is involved in a pathway downstream of PKA, this would result in the functional activation of LRRC72. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable analog of cAMP that activates PKA. Activation of PKA can lead to phosphorylation and activation of proteins within the cell, including LRRC72 if it is in the pathway or a substrate of PKA. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is an inhibitor of protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels of proteins within the cell. This could lead to the activation of LRRC72 by preventing its dephosphorylation and maintaining it in an active phosphorylated state. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Similar to Okadaic Acid, Calyculin A is also an inhibitor of protein phosphatases. By inhibiting these phosphatases, Calyculin A would contribute to the phosphorylation and consequent activation of proteins, potentially including LRRC72. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates stress-activated protein kinases such as JNK. If LRRC72 is a substrate for JNK or is involved in the JNK signaling pathway, the activation of this kinase by Anisomycin would lead to the activation of LRRC72. | ||||||
KN-93 | 139298-40-1 | sc-202199 | 1 mg | $178.00 | 25 | |
KN-93 is a known inhibitor of Ca2+/calmodulin-dependent protein kinase II (CaMKII). Inhibition of CaMKII can lead to compensatory activation of other pathways. If LRRC72 is part of a pathway that is compensatorily activated by the inhibition of CaMKII, then KN-93 could result in the functional activation of LRRC72. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is known to modulate kinase signaling pathways. Should LRRC72 be a part of or influenced by these pathways, the activity of EGCG on these kinases could lead to the phosphorylation and functional activation of LRRC72. | ||||||