LOC145814 Activators
Chemical activators of LOC145814 employ various intracellular signaling pathways to modify the activity of this protein. Calcium ionophore A23187 and Ionomycin function by increasing intracellular calcium levels; this rise in calcium concentration can activate calcium-dependent protein kinases that, in turn, phosphorylate LOC145814. Phorbol 12-myristate 13-acetate (PMA) and 1,2-Dioctanoyl-sn-glycerol mimic diacylglycerol, a physiological activator of protein kinase C (PKC). Upon activation, PKC phosphorylates an array of substrate proteins, including LOC145814. This phosphorylation can alter the protein's function, leading to its activation. Similarly, Forskolin boosts intracellular cyclic AMP (cAMP) by stimulating adenylyl cyclase, which activates protein kinase A (PKA). PKA then targets proteins such as LOC145814 for phosphorylation, thereby influencing their activity.
In addition to these direct activators, some chemicals modify the phosphorylation state of LOC145814 by inhibiting phosphatases that would otherwise dephosphorylate and deactivate the protein. Okadaic acid and Calyculin A are potent inhibitors of protein phosphatases 1 and 2A, which results in the sustained phosphorylation and thus, the activity of LOC145814. Thapsigargin disrupts calcium homeostasis by inhibiting sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium that can activate calcium-dependent kinases capable of phosphorylating LOC145814. Ouabain indirectly raises intracellular calcium by inhibiting Na+/K+ ATPase, which may result in the activation of LOC145814 via calcium-dependent kinases. Anisomycin activates stress-activated protein kinases, which could phosphorylate LOC145814. Bisindolylmaleimide I, though primarily a PKC inhibitor, may influence other kinase pathways that could lead to the activation of LOC145814. Finally, IBMX prevents the breakdown of cAMP by inhibiting phosphodiesterases, leading to sustained PKA activity and subsequent phosphorylation of target proteins such as LOC145814.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
Calcium ionophore A23187 allows calcium ions to enter the cell, thereby increasing intracellular calcium concentrations. Elevated calcium can activate calcium-dependent protein kinases, which in turn are involved in the phosphorylation and thus activation of LOC145814 as part of their downstream effects. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA acts as a diacylglycerol analog to activate protein kinase C (PKC). Activated PKC can phosphorylate a broad range of substrates, including proteins like LOC145814, leading to their functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin acts as an ionophore for calcium, similarly to A23187, increasing intracellular calcium concentration and activating calcium-dependent kinases that can phosphorylate and activate LOC145814. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 and 2A. By inhibiting these phosphatases, it prevents dephosphorylation of proteins, potentially maintaining LOC145814 in an activated, phosphorylated state. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium levels. The resulting activation of calcium-dependent kinases can lead to the phosphorylation and activation of LOC145814. | ||||||
Ouabain-d3 (Major) | sc-478417 | 1 mg | $516.00 | |||
Ouabain is an inhibitor of the Na+/K+ ATPase which leads to an indirect increase in intracellular calcium levels through modulation of the sodium-calcium exchanger. This increase in calcium can then activate calcium-dependent kinases that may phosphorylate and activate LOC145814. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, like Okadaic acid, is a potent inhibitor of certain protein phosphatases. This inhibition can lead to the accumulation of phosphorylated proteins, potentially including LOC145814, thus keeping it in an activated state. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is known to activate stress-activated protein kinases, which can lead to the phosphorylation and activation of a range of proteins, possibly including LOC145814. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $47.00 $254.00 | 2 | |
This synthetic analog of diacylglycerol activates protein kinase C (PKC), which can then phosphorylate and activate various proteins within the cell, potentially including LOC145814. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is a selective inhibitor of PKC, but it can also nonspecifically inhibit other kinases. Inhibition of these kinases may lead to compensatory activation of parallel pathways, potentially resulting in the activation of LOC145814 through cross-talk between signaling cascades. | ||||||