E1AF Activators
Forskolin, known for its ability to elevate intracellular cyclic AMP (cAMP), sets off a domino effect that activates protein kinase A (PKA), a catalyst for the phosphorylation of transcription factors that could enhance the expression or activity of E1AF. Similarly, dibutyryl-cAMP, a synthetic analog of cAMP, bypasses cellular receptors to directly stimulate PKA, potentially leading to E1AF upregulation. Retinoic acid enters the nucleus where it binds to retinoic acid receptors, influencing gene expression patterns including those that govern E1AF activation. Phorbol esters like PMA and TPA are robust activators of protein kinase C (PKC), which is intricately involved in several signaling pathways that can lead to an increase in E1AF transcription and activity. These esters are known for their role in the modulation of various proteins involved in cell growth and differentiation, which are processes where E1AF is a critical regulatory element.
Growth factors such as EGF trigger the activation of their respective receptors, setting in motion downstream signaling cascades that can culminate in the upregulation of E1AF. Histone deacetylase inhibitors like sodium butyrate relax the chromatin structure, making the genomic DNA more accessible for transcription and potentially facilitating an increase in E1AF expression. Calcium ionophores like ionomycin elevate intracellular calcium levels, which activate calmodulin-dependent kinases and might lead to an increase in E1AF. Lithium chloride, often associated with the Wnt signaling pathway, has implications for gene regulation that may include E1AF. Beta-adrenoceptor agonists such as isoproterenol increase cAMP levels, similar to forskolin, suggesting a possible route to E1AF activation through PKA. Small molecule inhibitors like LY294002 and U0126 target specific signaling kinases in the PI3K and MAPK/ERK pathways. By modulating these pathways, these inhibitors can lead to changes in the cell's regulatory landscape, which includes the expression or activity of E1AF.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin increases intracellular cAMP levels, which in turn can activate PKA. PKA can phosphorylate transcription factors that enhance E1AF expression or activity. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
As a cAMP analog, dibutyryl-cAMP can activate PKA directly, thereby potentially upregulating E1AF through enhanced transcription factor activation. | ||||||
Retinoic Acid, all trans | 302-79-4 | sc-200898 sc-200898A sc-200898B sc-200898C | 500 mg 5 g 10 g 100 g | $65.00 $319.00 $575.00 $998.00 | 28 | |
Retinoic acid modulates gene expression by activating retinoic acid receptors which can lead to the upregulation of E1AF. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC) which is involved in various signaling pathways that can enhance E1AF activity or expression. | ||||||
Sodium Butyrate | 156-54-7 | sc-202341 sc-202341B sc-202341A sc-202341C | 250 mg 5 g 25 g 500 g | $30.00 $46.00 $82.00 $218.00 | 19 | |
Sodium butyrate, a histone deacetylase inhibitor, can promote a more relaxed chromatin state, facilitating E1AF transcription. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin raises intracellular calcium levels, activating calmodulin-dependent kinases which can upregulate E1AF. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium can impact the Wnt signaling pathway, which has been associated with the regulation of genes like E1AF. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol, a beta-adrenoceptor agonist, raises cAMP levels, similarly leading to PKA activation and potential E1AF upregulation. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 inhibits PI3K, which can alter downstream signaling pathways, possibly resulting in increased E1AF activity. | ||||||
U-0126 | 109511-58-2 | sc-222395 sc-222395A | 1 mg 5 mg | $63.00 $241.00 | 136 | |
U0126 inhibits MEK, which may lead to altered ERK pathway signaling, potentially affecting E1AF expression. | ||||||