Zyxin Antibody (21): sc-136128

Zyxin Antibody (21) is a mouse monoclonal IgG1 kappa light chain antibody that detects Zyxin of avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Zyxin antibody (21) is available as the non-conjugated form. Zyxin is a low abundance phosphoprotein primarily localized to focal adhesion plaques, where Zyxin plays a crucial role in regulating cellular adhesion and signaling processes. Zyxin features multiple proline-rich sequences and three LIM domains, which are zinc finger domains that facilitate protein-protein interactions. Zyxin interacts with several key proteins at cell adhesion sites, including members of the cysteine-rich protein LIM domain-containing protein family, which are essential for maintaining the structural integrity of focal adhesions. The proline-rich domain of Zyxin has been shown to associate with the SH3 domain of p95 Vav, while Zyxin does not interact with similar SH3 domain-containing proteins such as GRB2 or PLCγ. Zyxin has been demonstrated to interact with the focal adhesion protein VASP, potentially aiding in the localization of VASP to focal adhesions and regions of high dynamic activity within the cell. Through these interactions, Zyxin may significantly contribute to the organization and regulation of the actin cytoskeleton in avian cells, highlighting Zyxin′s importance in cellular mechanics and signaling pathways.

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Zyxin Antibody (21) References:

1. Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton.  |  Sadler, I., et al. 1992. J Cell Biol. 119: 1573-87. PMID: 1469049
2. Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).  |  Reinhard, M., et al. 1995. Proc Natl Acad Sci U S A. 92: 7956-60. PMID: 7644520
3. SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin.  |  Hobert, O., et al. 1996. Oncogene. 12: 1577-81. PMID: 8622875
4. Molecular characterization of human zyxin.  |  Macalma, T., et al. 1996. J Biol Chem. 271: 31470-8. PMID: 8940160
5. Nuclear-cytoplasmic shuttling of the focal contact protein, zyxin: a potential mechanism for communication between sites of cell adhesion and the nucleus.  |  Nix, DA. and Beckerle, MC. 1997. J Cell Biol. 138: 1139-47. PMID: 9281590
6. Structural and functional similarities between the human cytoskeletal protein zyxin and the ActA protein of Listeria monocytogenes.  |  Golsteyn, RM., et al. 1997. J Cell Sci. 110 (Pt 16): 1893-906. PMID: 9296389

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Zyxin Antibody (21) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Zyxin Antibody (21) Specifications

Alternate Product Name: Zyxin

Product Clone Name: 21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 277-447

Epitope Target: Zyxin

Epitope Species: chicken

Product Reactivity/Detection: Zyxin

Species Reactivity/Detection: avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Zyxin Antibody (21): N/A

The preferred Zyxin antibody is Zyxin Antibody (2D1): sc-293448.