Zyxin Antibody (2D1): sc-293448

Zyxin Antibody (2D1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Zyxin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Zyxin antibody (2D1) is available as the non-conjugated form. Zyxin is a low abundance phosphoprotein primarily localized to focal adhesion plaques, where Zyxin plays a crucial role in regulating cellular adhesion and signaling processes. Zyxin′s structure includes multiple proline-rich sequences and three LIM domains, which are zinc finger domains that facilitate protein-protein interactions. These structural features are essential for Zyxin′s function, enabling interactions with various proteins at cell adhesion sites, including members of the cysteine-rich protein LIM domain-containing family. Notably, Zyxin′s proline-rich domain associates with the SH3 domain of p95 Vav, while showing no interaction with similar SH3 domains found in proteins like GRB2 or PLCg. Additionally, Zyxin interacts with the focal adhesion protein VASP, potentially aiding in VASP localization to focal adhesions and regions of high dynamic activity within the cell. Through these interactions, Zyxin contributes to the organization and dynamics of the actin cytoskeleton in mammalian cells, highlighting Zyxin′s importance in maintaining cellular integrity and facilitating cellular responses to external stimuli.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Zyxin Antibody (2D1) References:

1. Spatial proximity of proteins surrounding zyxin under force-bearing conditions.  |  Cheah, JS., et al. 2021. Mol Biol Cell. 32: 1221-1228. PMID: 33909446
2. Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression.  |  Yan, R., et al. 2021. Cell Death Dis. 12: 955. PMID: 34657146
3. Zyxin Mediates Vascular Repair via Endothelial Migration Promoted by Forskolin in Mice.  |  Kang, X., et al. 2021. Front Physiol. 12: 741699. PMID: 34690814
4. Cytoskeletal protein Zyxin in embryonic development: from controlling cell movements and pluripotency to regulating embryonic patterning.  |  Martynova, NY., et al. 2023. FEBS J. 290: 66-72. PMID: 34854244
5. Zyxin protects from hypertension-induced cardiac dysfunction.  |  Al-Hasani, J., et al. 2022. Cell Mol Life Sci. 79: 93. PMID: 35075545
6. Enhancement of Zyxin Promotes Skin Fibrosis by Regulating FAK/PI3K/AKT and TGF-β Signaling Pathways via Integrins.  |  Huang, Y., et al. 2023. Int J Biol Sci. 19: 2394-2408. PMID: 37215989
7. Zyxin inhibits the epithelial-mesenchymal transition process in gastric cancer by upregulating SIRT1.  |  Lou, J., et al. 2023. MedComm (2020). 4: e357. PMID: 37667739
8. Zyxin is important for the stability and function of podocytes, especially during mechanical stretch.  |  Kliewe, F., et al. 2024. Commun Biol. 7: 446. PMID: 38605154
9. The role of zyxin in signal transduction and its relationship with diseases.  |  Wu, Z., et al. 2024. Front Mol Biosci. 11: 1371549. PMID: 38712343
10. TBK1-Zyxin signaling controls tumor-associated macrophage recruitment to mitigate antitumor immunity.  |  Zhou, R., et al. 2024. EMBO J. 43: 4984-5017. PMID: 39304793

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Zyxin Antibody (2D1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Zyxin Antibody (2D1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Zyxin Antibody (2D1) Specifications

Alternate Product Name: Zyxin

Product Clone Name: 2D1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-572

Epitope Target: Zyxin

Epitope Species: human

Product Reactivity/Detection: Zyxin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Zyxin Antibody (2D1): N/A

The preferred Zyxin antibody is Zyxin Antibody (21): sc-136128.