UFD2 Antibody (C-1): sc-377072

UFD2 Antibody (C-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects UFD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UFD2 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UFD2, also known as ubiquitin conjugation factor E4 (UBE4B), plays a crucial role in the ubiquitin-proteasome system by binding to ubiquitin moieties of preformed conjugates and facilitating the assembly of ubiquitin chains in collaboration with E1, E2, and E3 enzymes. This process is vital for regulating protein degradation, which is essential for maintaining cellular homeostasis and responding to stress. During apoptosis, UFD2 undergoes proteolytic cleavage at Asp 123 by caspase-6 and granzyme B, with a significantly lower cleavage efficiency at Asp 109 by caspase-3 and caspase-7. UFD2′s E4 activity has been linked to cell survival under stress conditions in yeast, highlighting its importance in eukaryotic cellular functions. UFD2 is predominantly expressed in the ovary, testis, heart, and skeletal muscle, underscoring its potential significance in reproductive and muscular health.

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UFD2 Antibody (C-1) References:

1. A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly.  |  Koegl, M., et al. 1999. Cell. 96: 635-44. PMID: 10089879
2. A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration (WldS) mouse.  |  Conforti, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 11377-82. PMID: 11027338
3. Screening for gene mutations in a 500 kb neuroblastoma tumor suppressor candidate region in chromosome 1p; mutation and stage-specific expression in UBE4B/UFD2.  |  Krona, C., et al. 2003. Oncogene. 22: 2343-51. PMID: 12700669
4. Multiple interactions of rad23 suggest a mechanism for ubiquitylated substrate delivery important in proteolysis.  |  Kim, I., et al. 2004. Mol Biol Cell. 15: 3357-65. PMID: 15121879
5. Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage.  |  Saeki, Y., et al. 2004. Biochem Biophys Res Commun. 320: 840-5. PMID: 15240124
6. Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation.  |  Spinette, S., et al. 2004. Cell Cycle. 3: 1638-44. PMID: 15611659
7. Cdc48-Ufd2-Rad23: the road less ubiquitinated?  |  Bazirgan, OA. and Hampton, RY. 2005. Nat Cell Biol. 7: 207-9. PMID: 15738968
8. NAD and axon degeneration: from the Wlds gene to neurochemistry.  |  Wang, J. and He, Z. 2009. Cell Adh Migr. 3: 77-87. PMID: 19372760
9. Structural and functional characterization of the monomeric U-box domain from E4B.  |  Nordquist, KA., et al. 2010. Biochemistry. 49: 347-55. PMID: 20017557
10. Phosphorylations of Sds23/Psp1/Moc1 by stress-activated kinase and cAMP-dependent kinase are essential for regulating cell viability in prolonged stationary phase.  |  Jang, YJ., et al. 2013. Yeast. 30: 379-94. PMID: 23640764
11. Genome-wide analysis of the U-box E3 ubiquitin ligase enzyme gene family in tomato.  |  Sharma, B. and Taganna, J. 2020. Sci Rep. 10: 9581. PMID: 32533036
12. A heterotypic assembly mechanism regulates CHIP E3 ligase activity.  |  Das, A., et al. 2022. EMBO J. 41: e109566. PMID: 35762422

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UFD2 Antibody (C-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-UFD2 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UFD2 Antibody (C-1) Specifications

Alternate Product Name: UFD2

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 525-747

Epitope Target: UFD2

Epitope Species: human

Product Reactivity/Detection: UFD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for UFD2 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin