UBC13/UBE2NL Antibody (C-9): sc-365722

UBC13/UBE2NL Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBC13/UBE2NL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UBC13/UBE2NL Antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBC13, also known as UBE2N or BLU, is a critical component of the ubiquitin-proteasome system, which regulates protein degradation and cellular homeostasis. UBC13/UBE2NL (C-9) antibody targets proteins primarily located in the cytoplasm and nucleus, where UBC13 plays a vital role in mediating the synthesis of non-canonical polyubiquitin chains in conjunction with Mms2 partner. UBC13′s ability to form these chains is crucial for signaling pathways that do not lead to proteasomal degradation, thereby influencing various cellular processes such as DNA repair, cell cycle progression, and cellular differentiation. UBC13′s interaction with specific proteins, including E3 ligases, is essential for pathway regulation, highlighting UBC13′s importance in maintaining cellular integrity and responding to DNA damage. UBE2NL, a closely related protein encoded by a gene on human chromosome 12, shares functional similarities with UBC13, further emphasizing this protein family′s significance in cellular regulation.

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UBC13/UBE2NL Antibody (C-9) References:

1. RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO.  |  Hoege, C., et al. 2002. Nature. 419: 135-41. PMID: 12226657
2. Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A.  |  Andersen, PL., et al. 2005. J Cell Biol. 170: 745-55. PMID: 16129784
3. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.  |  Plans, V., et al. 2006. J Cell Biochem. 97: 572-82. PMID: 16215985
4. Key function for the Ubc13 E2 ubiquitin-conjugating enzyme in immune receptor signaling.  |  Yamamoto, M., et al. 2006. Nat Immunol. 7: 962-70. PMID: 16862162
5. A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination.  |  Zhao, GY., et al. 2007. Mol Cell. 25: 663-75. PMID: 17349954
6. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses.  |  Fukushima, T., et al. 2007. Proc Natl Acad Sci U S A. 104: 6371-6. PMID: 17404240
7. Substrate modification with lysine 63-linked ubiquitin chains through the UBC13-UEV1A ubiquitin-conjugating enzyme.  |  Petroski, MD., et al. 2007. J Biol Chem. 282: 29936-45. PMID: 17709375
8. Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.  |  Wang, B. and Elledge, SJ. 2007. Proc Natl Acad Sci U S A. 104: 20759-63. PMID: 18077395
9. Identification of New Lead Molecules Against UBE2NL Enzyme for Cancer Therapy.  |  Ramatenki, V., et al. 2017. Appl Biochem Biotechnol. 182: 1497-1517. PMID: 28185054
10. Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome.  |  Ni, J., et al. 2021. J Immunol. 206: 2376-2385. PMID: 33893171
11. A RAD18-UBC13-PALB2-RNF168 axis mediates replication fork recovery in BRCA1-deficient cancer cells.  |  Cybulla, E., et al. 2024. Nucleic Acids Res. 52: 8861-8879. PMID: 38943334
12. Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.  |  Yamaguchi, T., et al. 1996. J Biochem. 120: 494-97. PMID: 8902611

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UBC13/UBE2NL Antibody (C-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-UBC13/UBE2NL Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UBC13/UBE2NL Antibody (C-9) Specifications

Alternate Product Name: UBC13/UBE2NL

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-37

Epitope Target: UBC13

Epitope Species: human

Product Reactivity/Detection: UBC13 and UBE2NL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for UBC13/UBE2NL Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred UBC13 antibody is UBC13 Antibody (F-10): sc-376470.