UBC13 Antibody (F-10): sc-376470

UBC13 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBC13 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). UBC13 Antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBC13, also known as UBE2N or BLU, is a crucial member of the E2 ubiquitin-conjugating enzyme family, consisting of 152 amino acids. UBC13 plays a vital role in the ubiquitination process, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBC13 is unique in forming a heterodimer with Mms2 (UBE2V2) to catalyze the synthesis of non-canonical polyubiquitin chains, a process distinct from the typical proteasomal degradation pathway. This unique function allows for the regulation of various cellular processes, including DNA repair, cell cycle progression, and cellular differentiation, contributing to cell survival following DNA damage. UBC13′s ability to mediate the transcription of several target genes further underscores UBC13′s significance in cellular responses to stress and potential implications in cancer biology and therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

UBC13 Antibody (F-10) References:

1. RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO.  |  Hoege, C., et al. 2002. Nature. 419: 135-41. PMID: 12226657
2. Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A.  |  Andersen, PL., et al. 2005. J Cell Biol. 170: 745-55. PMID: 16129784
3. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.  |  Plans, V., et al. 2006. J Cell Biochem. 97: 572-82. PMID: 16215985
4. Key function for the Ubc13 E2 ubiquitin-conjugating enzyme in immune receptor signaling.  |  Yamamoto, M., et al. 2006. Nat Immunol. 7: 962-70. PMID: 16862162
5. A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination.  |  Zhao, GY., et al. 2007. Mol Cell. 25: 663-75. PMID: 17349954
6. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses.  |  Fukushima, T., et al. 2007. Proc Natl Acad Sci U S A. 104: 6371-6. PMID: 17404240
7. Substrate modification with lysine 63-linked ubiquitin chains through the UBC13-UEV1A ubiquitin-conjugating enzyme.  |  Petroski, MD., et al. 2007. J Biol Chem. 282: 29936-45. PMID: 17709375
8. Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.  |  Wang, B. and Elledge, SJ. 2007. Proc Natl Acad Sci U S A. 104: 20759-63. PMID: 18077395
9. Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome.  |  Ni, J., et al. 2021. J Immunol. 206: 2376-2385. PMID: 33893171
10. Functions and mechanisms of the Ubc13-UEV complex and lysine 63-linked polyubiquitination in plants.  |  Yang, K. and Xiao, W. 2022. J Exp Bot. 73: 5372-5387. PMID: 35640002
11. A RAD18-UBC13-PALB2-RNF168 axis mediates replication fork recovery in BRCA1-deficient cancer cells.  |  Cybulla, E., et al. 2024. Nucleic Acids Res. 52: 8861-8879. PMID: 38943334
12. Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.  |  Yamaguchi, T., et al. 1996. J Biochem. 120: 494-97. PMID: 8902611

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UBC13 Antibody (F-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-UBC13 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UBC13 Antibody (F-10) Specifications

Alternate Product Name: UBC13

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-75

Epitope Target: UBC13

Epitope Species: human

Product Reactivity/Detection: UBC13

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for UBC13 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred UBC13/UBE2NL antibody is UBC13/UBE2NL Antibody (C-9): sc-365722.