Date published: 2026-5-19
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TRβ1 Antibody (J52) - Western Blotting - Image 1665
TRβ1 Antibody (J52): sc-738. Western blot analysis of TRβ1 expression in C32 whole cell lysate.
TRβ1 Antibody (J52) - Western Blotting - Image 1665
TRβ1 Antibody (J52): sc-738. Western blot analysis of TRβ1 expression in C32 whole cell lysate.
TRβ1 Antibody (J52) - ChIP - Image 9368
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included TRβ1 (J51): sc-737 and TRβ1 Antibody (J52): sc-738 (B), p300 (C-20): sc-585, p300 (H-272): sc-8981 and p300 (N-15): sc-584 (C), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
TRβ1 Antibody (J52) - Western Blotting - Image 154021
TRβ1 Antibody (J52): sc-738. Western blot analysis of TRβ expression in non-transfected: sc-117752 (A) and human TRβ transfected: sc-369818 (B) 293T whole cell lysates.
TRβ1 Antibody (J52): sc-738. Western blot analysis of TRβ1 expression in C32 whole cell lysate.

TRβ1 Antibody (J52): sc-738

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Datasheets
  • TRβ1 Antibody (J52) is a mouse monoclonal IgG1 κ, cited in 45 publications, provided at 200 µg/ml
  • epitope mapping within the C-terminal half of the A/B domain of the TRβ1 of human origin
  • recommended for detection of TRβ1 of mouse, rat and human origin by WB, IP and IF
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-738 X, 200 µg/0.1 ml)
  • See TRβ1 (J51): sc-737 for TRβ1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRβ1 Antibody (J52) for WB applications. These reagents are now offered in bundles with TRβ1 Antibody (J52) (see ordering information below).
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Additional Thyroid Receptor Antibodies including TR α 1 and TR α 2

TRβ1 Antibody (J52) is a mouse monoclonal IgG1 antibody that detects TRβ1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TRβ1, a member of the thyroid hormone nuclear receptor family, plays a crucial role in mediating the effects of thyroid hormones on gene expression, which is vital for regulating metabolic processes, growth, and development. TRβ1 is primarily located in the nucleus, where TRβ1 functions as a ligand-dependent transcription factor, binding to thyroid hormone response elements (TREs) to modulate the transcription of target genes. Nuclear localization of TRβ1 is essential because TRβ1 allows for direct interaction with DNA and other transcriptional machinery, thereby influencing cellular responses to thyroid hormones. TRβ1 can form heterodimers with retinoid X receptors, enhancing transcriptional activity and allowing for a more nuanced regulation of gene expression in response to thyroid hormone levels. TRβ1′s ability to interact with various co-regulators and transcription factors underscores TRβ1′s importance in maintaining metabolic homeostasis and potential implications in thyroid-related disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

TRβ1 Antibody (J52) References:

  1. Cross-talk between signal transducer and activator of transcription (Stat5) and thyroid hormone receptor-beta 1 (TRbeta1) signaling pathways.  |  Favre-Young, H., et al. 2000. Mol Endocrinol. 14: 1411-24. PMID: 10976919
  2. Thyroid hormone receptor TRbeta1 mediates Akt activation by T3 in pancreatic beta cells.  |  Verga Falzacappa, C., et al. 2007. J Mol Endocrinol. 38: 221-33. PMID: 17293442
  3. The TRbeta1 is essential in mediating T3 action on Akt pathway in human pancreatic insulinoma cells.  |  Verga Falzacappa, C., et al. 2009. J Cell Biochem. 106: 835-48. PMID: 19160403
  4. Cytoplasm-to-nucleus shuttling of thyroid hormone receptor-beta1 (Trbeta1) is directed from a plasma membrane integrin receptor by thyroid hormone.  |  Cao, HJ., et al. 2009. Endocr Res. 34: 31-42. PMID: 19557589
  5. Dose and time-dependent hypercholesterolemic effects of iodine excess via TRbeta1-mediated down regulation of hepatic LDLr gene expression.  |  Zhao, LN., et al. 2010. Eur J Nutr. 49: 257-65. PMID: 19916081
  6. Novel mechanism of positive versus negative regulation by thyroid hormone receptor β1 (TRβ1) identified by genome-wide profiling of binding sites in mouse liver.  |  Ramadoss, P., et al. 2014. J Biol Chem. 289: 1313-28. PMID: 24288132
  7. The two major isoforms of thyroid hormone receptor, TRα1 and TRβ1, preferentially partner with distinct panels of auxiliary proteins.  |  Hahm, JB., et al. 2014. Mol Cell Endocrinol. 383: 80-95. PMID: 24325866
  8. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.  |  Peng, X., et al. 2018. Mol Cell Biochem. 449: 207-218. PMID: 29679278
  9. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells.  |  Peng, X., et al. 2018. Oncol Lett. 16: 785-792. PMID: 29963146
  10. Relationship between the expression of TRβ1 and the molecular typing and clinicopathological features of breast cancer.  |  Miao, Y., et al. 2017. Int J Clin Exp Pathol. 10: 8875-8883. PMID: 31966755

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

TRβ1 Antibody (J52)

sc-738
200 µg/ml
$322.00

TRβ1 Antibody (J52): m-IgG Fc BP-HRP Bundle

sc-536541
200 µg Ab; 10 µg BP
$361.00

TRβ1 Antibody (J52): m-IgGκ BP-HRP Bundle

sc-533692
200 µg Ab; 40 µg BP
$361.00

TRβ1 Antibody (J52): m-IgG1 BP-HRP Bundle

sc-544664
200 µg Ab; 20 µg BP
$361.00

TRβ1 Antibody (J52) X

sc-738 X
200 µg/0.1 ml
$322.00

When should the TransCruz reagent sc-738 X be used instead of the standard form sc-738?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from The best choice for this target.No need to choose another antibody for TR beta 1 detection. This antibody works well in mouse, rat, and human samples and provides great WB data. It has been published with over 30 times.
Date published: 2017-01-27
Rated 5 out of 5 by from wonderful bands in WBcleaner，wonderful bands in WB，and testing in Rat Liver
Date published: 2016-12-06
Rated 4 out of 5 by from Clean band in western blot analysis of TRClean band in western blot analysis of TR 1 expression in C32 whole cell lysate. -SCBT QC
Date published: 2015-05-27
Rated 4 out of 5 by from Used for ChIP with CacoUsed for ChIP with Caco-2 cells (intestinal epithelial cell lines). -SCBT Publication Review
Date published: 2015-05-08
Rated 4 out of 5 by from Published IHC data using paraffin embeddedPublished IHC data using paraffin embedded rat liver samples. -SCBT Publication Review
Date published: 2015-03-28
Rated 5 out of 5 by from 1:500 dilution used for western blotting1:500 dilution used for western blotting with murine heart samples. -SCBT Publication Review
Date published: 2015-02-01
Rated 5 out of 5 by from 1:1000 dilution used for western blotting1:1000 dilution used for western blotting with murine astrocytes. -SCBT Publication Review
Date published: 2014-10-19
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TRβ1 Antibody (J52) is rated 4.6 out of 5 by 7.
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