TRβ1 Antibody (J51): sc-737

TRβ1 Antibody (J51) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRβ1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin embedded sections (IHCP). TRβ1 Antibody (J51) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. TRβ1, a member of the thyroid hormone nuclear receptor family, plays a crucial role in mediating the effects of thyroid hormones on growth, differentiation, and metabolism. Located primarily in the nucleus, TRβ1 functions as a ligand-dependent transcription factor that regulates gene expression by binding to thyroid hormone response elements (TREs) in the DNA. This binding is essential for the proper transcriptional regulation of genes involved in metabolic processes and development. TRβ1 isoform is particularly important in pancreatic β cells, where TRβ1 interacts with the p85α subunit of phosphoinositide 3-kinase, facilitating the T3-induced activation of Akt, a key signaling pathway that influences insulin secretion and glucose homeostasis. TRβ1′s ability to heterodimerize with retinoid X receptors further enhances regulatory capacity, making TRβ1 a vital component in the intricate network of hormonal signaling and metabolic regulation.

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TRβ1 Antibody (J51) References:

1. The orientation and spacing of core DNA-binding motifs dictate selective transcriptional responses to three nuclear receptors.  |  Näär, AM., et al. 1991. Cell. 65: 1267-79. PMID: 1648451
2. Thyroid hormone receptor TRbeta1 mediates Akt activation by T3 in pancreatic beta cells.  |  Verga Falzacappa, C., et al. 2007. J Mol Endocrinol. 38: 221-33. PMID: 17293442
3. T3 enhances thyroid cancer cell proliferation through TRβ1/Oct-1-mediated cyclin D1 activation.  |  Perri, A., et al. 2014. Mol Cell Endocrinol. 382: 205-217. PMID: 24121026
4. Hepatic nuclear corepressor 1 regulates cholesterol absorption through a TRβ1-governed pathway.  |  Astapova, I., et al. 2014. J Clin Invest. 124: 1976-86. PMID: 24713658
5. Subchronic Exposure to Arsenic Represses the TH/TRβ1-CaMK IV Signaling Pathway in Mouse Cerebellum.  |  Guan, H., et al. 2016. Int J Mol Sci. 17: PMID: 26821021
6. Suppression of Calcium Entry Modulates the Expression of TRβ1 and Runx2 in Thyroid Cancer Cells, Two Transcription Factors That Regulate Invasion, Proliferation and Thyroid-Specific Protein Levels.  |  Asghar, MY., et al. 2022. Cancers (Basel). 14: PMID: 36497320
7. Biphasic expression of thyroid hormone receptor TRβ1 in mammalian retina and anterior ocular tissues.  |  Ng, L., et al. 2023. Front Endocrinol (Lausanne). 14: 1174600. PMID: 37033230
8. Phosphorylation enhances the target gene sequence-dependent dimerization of thyroid hormone receptor with retinoid X receptor.  |  Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736
9. Hetero- and homodimeric receptors in thyroid hormone and vitamin A action.  |  Zhang, XK. and Pfahl, M. 1993. Receptor. 3: 183-91. PMID: 8167569
10. Phosphorylation selectively increases triiodothyronine receptor homodimer binding to DNA.  |  Sugawara, A., et al. 1994. J Biol Chem. 269: 433-7. PMID: 8276832
11. Thyroid hormone receptors: multiple forms, multiple possibilities.  |  Lazar, MA. 1993. Endocr Rev. 14: 184-93. PMID: 8325251
12. Interaction of human beta 1 thyroid hormone receptor and its mutants with DNA and retinoid X receptor beta. T3 response element-dependent dominant negative potency.  |  Meier, CA., et al. 1993. J Clin Invest. 92: 1986-93. PMID: 8408652

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRβ1 Antibody (J51) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-TRβ1 Antibody (J51) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRβ1 Antibody (J51) Specifications

Alternate Product Name: TRβ1

Product Clone Name: J51

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TRβ1

Epitope Species: human

Product Reactivity/Detection: TRβ1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for TRβ1 Antibody (J51): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent