TORC2 Antibody (E-10): sc-515488
- TORC2 Antibody (E-10) is a mouse monoclonal IgG1 κ TORC2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 566-590 near the C-terminus of TORC2 of human origin
- TORC2 Antibody (E-10) is recommended for detection of TORC2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TORC2 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515488 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TORC2 Antibody (E-10) for WB applications. These reagents are now offered in bundles with TORC2 Antibody (E-10) (see ordering information below).
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TORC2 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TORC2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TORC2 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. TORC2, part of the transducer of regulated cAMP response element-binding family, plays a crucial role in cellular signaling by acting as a coactivator for the cAMP response element-binding protein (CREB). This interaction regulates gene expression in response to hormonal signals, particularly those mediated by cAMP. TORC2′s ability to translocate from the nucleus to the cytoplasm in a CRM1-dependent manner serves as a key regulatory mechanism influencing activity and function. Understanding TORC2′s localization dynamics and phosphorylation-dependent interactions helps elucidate roles in various physiological processes and potential implications in diseases where cAMP signaling is disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TORC2 Antibody (E-10) References:
- The CREB coactivator TORC2 is a key regulator of fasting glucose metabolism. | Koo, SH., et al. 2005. Nature. 437: 1109-11. PMID: 16148943
- Dual role of the coactivator TORC2 in modulating hepatic glucose output and insulin signaling. | Canettieri, G., et al. 2005. Cell Metab. 2: 331-8. PMID: 16271533
- Single nucleotide polymorphisms in genes encoding LKB1 (STK11), TORC2 (CRTC2) and AMPK alpha2-subunit (PRKAA2) and risk of type 2 diabetes. | Keshavarz, P., et al. 2008. Mol Genet Metab. 93: 200-9. PMID: 17950019
- Essential function of TORC2 in PKC and Akt turn motif phosphorylation, maturation and signalling. | Ikenoue, T., et al. 2008. EMBO J. 27: 1919-31. PMID: 18566587
- Palomid 529, a novel small-molecule drug, is a TORC1/TORC2 inhibitor that reduces tumor growth, tumor angiogenesis, and vascular permeability. | Xue, Q., et al. 2008. Cancer Res. 68: 9551-7. PMID: 19010932
- TORC2 regulates hepatic insulin signaling via a mammalian phosphatidic acid phosphatase, LIPIN1. | Ryu, D., et al. 2009. Cell Metab. 9: 240-51. PMID: 19254569
- The induction of IL-10 by zymosan in dendritic cells depends on CREB activation by the coactivators CREB-binding protein and TORC2 and autocrine PGE2. | Alvarez, Y., et al. 2009. J Immunol. 183: 1471-9. PMID: 19564345
- Novel liver-specific TORC2 siRNA corrects hyperglycemia in rodent models of type 2 diabetes. | Saberi, M., et al. 2009. Am J Physiol Endocrinol Metab. 297: E1137-46. PMID: 19706791
- Co-administration of the mTORC1/TORC2 inhibitor INK128 and the Bcl-2/Bcl-xL antagonist ABT-737 kills human myeloid leukemia cells through Mcl-1 down-regulation and AKT inactivation. | Rahmani, M., et al. 2015. Haematologica. 100: 1553-63. PMID: 26452980
- SHORT syndrome due to a novel de novo mutation in PRKCE (Protein Kinase Cɛ) impairing TORC2-dependent AKT activation. | Alcantara, D., et al. 2017. Hum Mol Genet. 26: 3713-3721. PMID: 28934384
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TORC2 Antibody (E-10) | sc-515488 | 200 µg/ml | $316.00 | |||
TORC2 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-531389 | 200 µg Ab; 10 µg BP | $354.00 | |||
TORC2 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-525067 | 200 µg Ab, 40 µg BP | $354.00 | |||
TORC2 Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-544522 | 200 µg Ab; 20 µg BP | $354.00 | |||
TORC2 Antibody (E-10) X | sc-515488 X | 200 µg/0.1 ml | $316.00 | |||
TORC2 Antibody (E-10) AC | sc-515488 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TORC2 Antibody (E-10) HRP | sc-515488 HRP | 200 µg/ml | $316.00 | |||
TORC2 Antibody (E-10) FITC | sc-515488 FITC | 200 µg/ml | $330.00 | |||
TORC2 Antibody (E-10) PE | sc-515488 PE | 200 µg/ml | $343.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 488 | sc-515488 AF488 | 200 µg/ml | $357.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 546 | sc-515488 AF546 | 200 µg/ml | $357.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 594 | sc-515488 AF594 | 200 µg/ml | $357.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 647 | sc-515488 AF647 | 200 µg/ml | $357.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 680 | sc-515488 AF680 | 200 µg/ml | $357.00 | |||
TORC2 Antibody (E-10) Alexa Fluor® 790 | sc-515488 AF790 | 200 µg/ml | $357.00 | |||
TORC2 (E-10) Neutralizing Peptide | sc-515488 P | 100 µg/0.5 ml | $68.00 |