Date published: 2025-10-16
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Titin Antibody (E-4) - Immunofluorescence - Image 142798
Titin Antibody (E-4): sc-271945. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Titin Antibody (E-4) - Immunofluorescence - Image 142798
Titin Antibody (E-4): sc-271945. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Titin Antibody (E-4) - Western Blotting - Image 145989
Dot blot analysis of Titin expression in A-10 whole cell lysate immunoprecipitated with Titin (H-300): sc-28536 and detected with Titin Antibody (E-4): sc-271945.
Titin Antibody (E-4): sc-271945. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Titin Antibody (E-4): sc-271945

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Datasheets
  • Titin Antibody (E-4) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 33124-33423 mapping at the C-terminus of Titin of human origin
  • recommended for detection of Titin of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Titin Antibody (E-4) for WB applications. These reagents are now offered in bundles with Titin Antibody (E-4) (see ordering information below).

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Research Information

SEE ALSO...

Titin Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects Titin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Titin antibody (E-4) targets a giant protein composed of 27,000 amino acids, which plays a crucial role in the assembly and maintenance of sarcomeres, the fundamental contractile units of striated muscle. Titin (E-4) monoclonal antibody recognizes a protein essential for the structural integrity of myofibrils during muscle contraction and participates in organizing the machinery necessary for chromosome condensation during cell division. The unique structure of Titin includes an autoregulated serine kinase catalytic domain and a calcium/calmodulin binding region, which are vital for activation and function. Upon activation, Titin phosphorylates telethonin, a sarcomeric protein abundant in both heart and skeletal muscle, highlighting a significant role in cytoskeletal reorganization during myofibrillogenesis. Titin′s interaction with various proteins underscores its importance in muscle physiology and cellular processes, making anti-Titin antibody (E-4) an invaluable tool for researchers studying muscle development and related disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Titin Antibody (E-4) References:

  1. Cardiac titin and heart disease.  |  LeWinter, MM. and Granzier, HL. 2014. J Cardiovasc Pharmacol. 63: 207-12. PMID: 24072177
  2. Softening the Stressed Giant Titin in Diabetes Mellitus.  |  Methawasin, M. and Granzier, H. 2018. Circ Res. 123: 315-317. PMID: 30026374
  3. Titin Truncation Variant in Dilated Cardiomyopathy.  |  Ito, M. and Morita, H. 2021. Int Heart J. 62: 221-223. PMID: 33790133
  4. Truncated titin proteins and titin haploinsufficiency are targets for functional recovery in human cardiomyopathy due to TTN mutations.  |  Fomin, A., et al. 2021. Sci Transl Med. 13: eabd3079. PMID: 34731013
  5. Truncated titin proteins in dilated cardiomyopathy.  |  McAfee, Q., et al. 2021. Sci Transl Med. 13: eabd7287. PMID: 34731015
  6. Advances in understanding the state of titin truncation variants in dilated cardiomyopathy.  |  Solaro, RJ. 2022. Pflugers Arch. 474: 265-266. PMID: 35020065
  7. Stretching the story of titin and muscle function.  |  Linke, WA. 2023. J Biomech. 152: 111553. PMID: 36989971
  8. Titin copy number variations associated with dominant inherited phenotypes.  |  Perrin, A., et al. 2024. J Med Genet. 61: 369-377. PMID: 37935568
  9. Titin-Based Force Modulates Cardiac Thick and Thin Filaments.  |  Hessel, AL., et al. 2024. Circ Res. 134: 1026-1028. PMID: 38482667
  10. Titin: roles in cardiac function and diseases.  |  Stroik, D., et al. 2024. Front Physiol. 15: 1385821. PMID: 38660537

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Titin Antibody (E-4)

sc-271945
200 µg/ml
$316.00

Titin Antibody (E-4): m-IgG Fc BP-HRP Bundle

sc-537711
200 µg Ab; 10 µg BP
$354.00

Titin Antibody (E-4): m-IgGκ BP-HRP Bundle

sc-535069
200 µg Ab; 40 µg BP
$354.00

Titin Antibody (E-4): m-IgG1 BP-HRP Bundle

sc-545320
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with Titin (E-4): sc-271945 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good signalTitin Antibody (E-4) worked well in Western blot and in IF. The results were clear with little to no background
Date published: 2017-01-30
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-09
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Titin Antibody (E-4) is rated 5.0 out of 5 by 3.
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  • cp_1, bvpage1
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