Date published: 2025-12-18
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Titin Antibody (E-2) - Western Blotting - Image 135053
Dot blot analysis of Titin expression in A-10 (A), SJRH30 (B) and Sol8 (C) whole cell lysates immunoprecipitated with Titin (N-20): sc-8723 and detected with Titin Antibody (E-2): sc-271946.
Dot blot analysis of Titin expression in A-10 (A), SJRH30 (B) and Sol8 (C) whole cell lysates immunoprecipitated with Titin (N-20): sc-8723 and detected with Titin Antibody (E-2): sc-271946.

Titin Antibody (E-2): sc-271946

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Datasheets
  • Titin Antibody (E-2) is a mouse monoclonal IgG3 κ Titin antibody, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 33124-33423 mapping at the C-terminus of Titin of human origin
  • Titin Antibody (E-2) is recommended for detection of Titin of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-Titin Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG3 BP-HRP is the preferred secondary detection reagent for Titin Antibody (E-2) for WB applications. This reagent is now offered in a bundle with Titin Antibody (E-2) (see ordering information below).

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Research Information

SEE ALSO...

Titin Antibody (E-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects Titin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Titin antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Titin, also known as connectin, is a colossal protein that plays a crucial role in the assembly and maintenance of sarcomeres, the fundamental contractile units of striated muscle. Titin′s unique structure, comprising approximately 27,000 amino acids, allows Titin to span half the length of a sarcomere, providing elasticity and structural support during muscle contraction. This structural integrity is vital for muscle function, as Titin helps prevent damage during the mechanical stress of contraction and relaxation cycles. Additionally, Titin contains an autoregulated serine kinase domain and a calcium/calmodulin binding region, which are essential for activation and regulation. Titin′s phosphorylation of telethonin, a sarcomeric protein, further highlights Titin′s importance in the reorganization of the cytoskeleton during myofibrillogenesis, linking Titin′s activity to both muscle development and cellular integrity.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Titin Antibody (E-2) References:

  1. Muscle assembly: a titanic achievement?  |  Gregorio, CC., et al. 1999. Curr Opin Cell Biol. 11: 18-25. PMID: 10047523
  2. Titin: a molecular control freak.  |  Trinick, J. and Tskhovrebova, L. 1999. Trends Cell Biol. 9: 377-80. PMID: 10481174
  3. Regulation of the actin-myosin interaction by titin.  |  Niederländer, N., et al. 2004. Eur J Biochem. 271: 4572-81. PMID: 15560799
  4. Calcium transients regulate titin organization during myofibrillogenesis.  |  Harris, BN., et al. 2005. Cell Motil Cytoskeleton. 60: 129-39. PMID: 15662726
  5. Phosphorylation of titin modulates passive stiffness of cardiac muscle in a titin isoform-dependent manner.  |  Fukuda, N., et al. 2005. J Gen Physiol. 125: 257-71. PMID: 15738048
  6. Truncations of titin causing dilated cardiomyopathy.  |  Herman, DS., et al. 2012. N Engl J Med. 366: 619-28. PMID: 22335739
  7. Recessive truncating titin gene, TTN, mutations presenting as centronuclear myopathy.  |  Ceyhan-Birsoy, O., et al. 2013. Neurology. 81: 1205-14. PMID: 23975875
  8. Titin mutations and muscle disease.  |  Kellermayer, D., et al. 2019. Pflugers Arch. 471: 673-682. PMID: 30919088
  9. Titin as a scaffold and spring. Cytoskeleton.  |  Trinick, J. 1996. Curr Biol. 6: 258-60. PMID: 8805236
  10. Telethonin, a novel sarcomeric protein of heart and skeletal muscle.  |  Valle, G., et al. 1997. FEBS Lett. 415: 163-8. PMID: 9350988
  11. The clash in titin.  |  Means, AR. 1998. Nature. 395: 846-7. PMID: 9804415
  12. Structural basis for activation of the titin kinase domain during myofibrillogenesis.  |  Mayans, O., et al. 1998. Nature. 395: 863-9. PMID: 9804419

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Titin Antibody (E-2)

sc-271946
200 µg/ml
$316.00

Titin Antibody (E-2): m-IgG3 BP-HRP Bundle

sc-550564
200 µg Ab; 40 µg BP
$354.00

Titin Antibody (E-2) AC

sc-271946 AC
500 µg/ml, 25% agarose
$416.00

Titin Antibody (E-2) HRP

sc-271946 HRP
200 µg/ml
$316.00

Titin Antibody (E-2) FITC

sc-271946 FITC
200 µg/ml
$330.00

Titin Antibody (E-2) PE

sc-271946 PE
200 µg/ml
$343.00

Titin Antibody (E-2) Alexa Fluor® 488

sc-271946 AF488
200 µg/ml
$357.00

Titin Antibody (E-2) Alexa Fluor® 546

sc-271946 AF546
200 µg/ml
$357.00

Titin Antibody (E-2) Alexa Fluor® 594

sc-271946 AF594
200 µg/ml
$357.00

Titin Antibody (E-2) Alexa Fluor® 647

sc-271946 AF647
200 µg/ml
$357.00

Titin Antibody (E-2) Alexa Fluor® 680

sc-271946 AF680
200 µg/ml
$357.00

Titin Antibody (E-2) Alexa Fluor® 790

sc-271946 AF790
200 µg/ml
$357.00

Are there isotype control products available for the IP of this product?

Asked by: kkk0033
Thank you for your question. The recommended isotype control is normal mouse IgG3: sc-3880.
Answered by: Technical Service SH
Date published: 2024-12-16

I am considering purchasing this antibody. Has it been used for immunoprecipitation (IP) in mouse tissues? If there are any laboratory data available, I would appreciate it if you could share them with me.

Asked by: kkk0033
Thank you very much for your question.Yes the antibody can be used for IP experiments in mice. Warranty service is available. Thank you.
Answered by: Jiajun Z
Date published: 2024-12-12

What is the recommended fixation method for immunofluorescence staining with Titin (E-2): sc-271946 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from weak signalI used it in IF on human ipsc-derived cardiomyocytes at 1:200 obtaining a very weak signal
Date published: 2022-06-15
Rated 4 out of 5 by from Great resultsThe results are better than what I expected, and meet the requirement of publication.
Date published: 2017-11-08
Rated 3 out of 5 by from 没有杂出来我一个月前申请了该抗体的试用装，很高兴可以提供试用反馈，这个抗体我没有杂出来满意的条带，也可能跟我的实验条件有关系，我继续优化一下条件试试看。
Date published: 2017-09-27
Rated 5 out of 5 by from Strong and specificGreat choice if you're looking for a reliable and clean monoclonal antibody to detect Titin in WB or IHC.
Date published: 2017-01-31
Rated 5 out of 5 by from Produced exceptional immunofluorescence membraneProduced exceptional immunofluorescence membrane and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-21
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Titin Antibody (E-2) is rated 4.0 out of 5 by 5.
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