Titin Inhibitors
The chemical class of Titin inhibitors comprises a diverse range of compounds that modulate Titin function within the sarcomere, impacting its mechanical interactions and contractile properties. Among these inhibitors, Blebbistatin directly targets the actomyosin interaction, disrupting the cross-bridge cycle and leading to decreased contractility and tension in the sarcomere. GNE-3511 is another direct inhibitor that disrupts the Titin-actin interaction, impairing sarcomere function and reducing Titin-mediated elasticity. In contrast, several compounds, including ML-7 and Y-27632, act indirectly by targeting the RhoA/ROCK signaling pathway. These inhibitors reduce myosin light chain phosphorylation, affecting actomyosin interaction and contractility, consequently influencing Titin function.
CK-666, Cytochalasin D, and Latrunculin A indirectly impact Titin by modulating actin polymerization dynamics. CK-666 inhibits the Arp2/3 complex, impairing actin filament branching, while Cytochalasin D and Latrunculin A disrupt actin polymerization, altering the actin cytoskeleton and indirectly affecting Titin's mechanical interactions. Bleomycin, Calyculin A, and Jasplakinolide exert their effects on Titin indirectly through diverse mechanisms. Bleomycin induces DNA damage, activating the DNA damage response, which influences cellular contractility. Calyculin A inhibits protein phosphatases, altering Titin's phosphorylation state. Jasplakinolide stabilizes actin filaments, indirectly influencing Titin-mediated sarcomere dynamics. Finally, BAPTA-AM chelates intracellular calcium ions, indirectly modulating Titin function by influencing calcium-dependent processes. Collectively, these Titin inhibitors provide valuable tools for investigating the intricate molecular mechanisms underlying sarcomere dynamics and contractility.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
(±)-Blebbistatin | 674289-55-5 | sc-203532B sc-203532 sc-203532A sc-203532C sc-203532D | 5 mg 10 mg 25 mg 50 mg 100 mg | $183.00 $313.00 $464.00 $942.00 $1723.00 | 7 | |
Blebbistatin acts as a direct inhibitor of Titin by targeting the actomyosin interaction. It binds to myosin ATPase, inhibiting the ATPase activity and disrupting the actomyosin cross-bridge cycle. This disruption results in decreased contractility and tension in the sarcomere, ultimately leading to Titin inhibition. | ||||||
ML-7 hydrochloride | 110448-33-4 | sc-200557 sc-200557A | 10 mg 50 mg | $91.00 $267.00 | 13 | |
ML-7 functions as an indirect Titin inhibitor by modulating the RhoA/ROCK signaling pathway. It inhibits ROCK, leading to decreased phosphorylation of myosin light chain (MLC). The reduced MLC phosphorylation affects the actomyosin interaction and contractility, indirectly influencing Titin function within the sarcomere. | ||||||
Y-27632, free base | 146986-50-7 | sc-3536 sc-3536A | 5 mg 50 mg | $186.00 $707.00 | 88 | |
Y-27632 acts as an indirect Titin inhibitor by targeting the RhoA/ROCK signaling pathway. It inhibits ROCK, resulting in reduced phosphorylation of myosin light chain (MLC). The decreased MLC phosphorylation affects the actomyosin interaction and contractility, indirectly influencing Titin function within the sarcomere. | ||||||
CK 666 | 442633-00-3 | sc-361151 sc-361151A | 10 mg 50 mg | $321.00 $1040.00 | 5 | |
CK-666 serves as an indirect Titin inhibitor by modulating the Arp2/3 complex-mediated actin polymerization. It inhibits Arp2/3, leading to impaired actin filament branching and dynamics. The altered actin cytoskeleton indirectly affects Titin's mechanical interactions, contributing to Titin inhibition within the sarcomere. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $165.00 $486.00 | 64 | |
Cytochalasin D acts as an indirect Titin inhibitor by disrupting actin polymerization. It binds to the growing ends of actin filaments, preventing further polymerization. The altered actin cytoskeleton and reduced actin dynamics indirectly affect Titin's mechanical interactions, contributing to Titin inhibition within the sarcomere. | ||||||
Latrunculin A, Latrunculia magnifica | 76343-93-6 | sc-202691 sc-202691B | 100 µg 500 µg | $265.00 $815.00 | 36 | |
Latrunculin A functions as an indirect Titin inhibitor by disrupting actin polymerization. It sequesters monomeric actin, preventing its incorporation into actin filaments. The altered actin cytoskeleton and reduced actin dynamics indirectly affect Titin's mechanical interactions, contributing to Titin inhibition within the sarcomere. | ||||||
Bleomycin | 11056-06-7 | sc-507293 | 5 mg | $275.00 | 5 | |
Bleomycin acts as an indirect Titin inhibitor by inducing DNA damage and activating the DNA damage response. The DNA damage response influences cytoskeletal dynamics and cellular contractility, indirectly affecting Titin function within the sarcomere. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A serves as an indirect Titin inhibitor by modulating protein phosphatase activity. It inhibits protein phosphatases, leading to increased phosphorylation of cytoskeletal proteins, including Titin. The altered phosphorylation state influences Titin's mechanical interactions and function within the sarcomere. | ||||||
Jasplakinolide | 102396-24-7 | sc-202191 sc-202191A | 50 µg 100 µg | $184.00 $305.00 | 59 | |
Jasplakinolide functions as an indirect Titin inhibitor by stabilizing actin filaments and preventing depolymerization. The stabilized actin cytoskeleton indirectly affects Titin's mechanical interactions, contributing to Titin inhibition within the sarcomere. | ||||||
BAPTA/AM | 126150-97-8 | sc-202488 sc-202488A | 25 mg 100 mg | $138.00 $458.00 | 61 | |
BAPTA-AM acts as an indirect Titin inhibitor by chelating intracellular calcium ions. The reduction in intracellular calcium levels influences calcium-dependent processes, including Titin-mediated sarcomere dynamics. This indirect modulation contributes to Titin inhibition within the sarcomere. | ||||||