Tie-2 Antibody (3A5): sc-293414

Tie-2 Antibody (3A5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tie-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Tie-2 antibody (3A5) is available as the non-conjugated form, which is crucial for studying the role of receptor tyrosine kinases in various biological processes, particularly in the vascular system. Tie-2 (3A5) antibody recognizes Tie-2, also known as Tek, which is primarily located on the surface of endothelial cells and plays a vital role in angiogenesis and vascular stability. Tie-2 (3A5) monoclonal antibody enables research into the regulation of blood vessel formation and maintenance, as Tie-2 mediates signaling pathways that influence endothelial cell survival, proliferation, and migration. Dysregulation of Tie-2 signaling has been implicated in various pathological conditions, including cancer and cardiovascular diseases, making Tie-2 a significant target for therapeutic intervention. Anti-Tie-2 antibody (3A5) helps researchers study Tie-2′s interactions with several ligands, including angiopoietins, which are critical for promoting vascular integrity and remodeling. Tie-2 (3A5) monoclonal antibody provides valuable insights into the mechanisms of vascular development and potential implications of Tie-2 in disease states.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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Tie-2 Antibody (3A5) References:

1. The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor.  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
2. A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains.  |  Partanen, J., et al. 1992. Mol Cell Biol. 12: 1698-707. PMID: 1312667
3. tek, a novel tyrosine kinase gene located on mouse chromosome 4, is expressed in endothelial cells and their presumptive precursors.  |  Dumont, DJ., et al. 1992. Oncogene. 7: 1471-80. PMID: 1630810
4. Localization of Ang-1, -2, Tie-2, and VEGF expression at endothelial-pericyte interdigitation in rat angiogenesis.  |  Wakui, S., et al. 2006. Lab Invest. 86: 1172-84. PMID: 16969369
5. Receptor tyrosine kinases: genetic evidence for their role in Drosophila and mouse development.  |  Pawson, T. and Bernstein, A. 1990. Trends Genet. 6: 350-6. PMID: 1965067
6. Placental mRNA expression of angiopoietins (Ang)-1, Ang-2 and their receptor Tie-2 is altered in pregnancies complicated by preeclampsia.  |  Kappou, D., et al. 2014. Placenta. 35: 718-23. PMID: 25047691
7. T11TS inhibits Angiopoietin-1/Tie-2 signaling, EGFR activation and Raf/MEK/ERK pathway in brain endothelial cells restraining angiogenesis in glioma model.  |  Bhattacharya, D., et al. 2015. Exp Mol Pathol. 98: 455-66. PMID: 25797371
8. Lack of FADD in Tie-2 expressing cells causes RIPK3-mediated embryonic lethality.  |  Fan, C., et al. 2016. Cell Death Dis. 7: e2351. PMID: 27584790
9. Expression and significance of B7-H3 and Tie-2 in the tumor vasculature of clear cell renal carcinoma.  |  Zhang, X., et al. 2017. Onco Targets Ther. 10: 5417-5424. PMID: 29180874
10. Hyperoxia-induced S1P1 signaling reduced angiogenesis by suppression of TIE-2 leading to experimental bronchopulmonary dysplasia.  |  Sudhadevi, T., et al. 2021. Cell Biochem Biophys. 79: 561-573. PMID: 34176100
11. The endothelial-specific receptor tyrosine kinase, tek, is a member of a new subfamily of receptors.  |  Dumont, DJ., et al. 1993. Oncogene. 8: 1293-301. PMID: 8386827
12. Tie-1 and tie-2 define another class of putative receptor tyrosine kinase genes expressed in early embryonic vascular system.  |  Sato, TN., et al. 1993. Proc Natl Acad Sci U S A. 90: 9355-8. PMID: 8415706

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tie-2 Antibody (3A5) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Tie-2 Antibody (3A5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tie-2 Antibody (3A5) Specifications

Alternate Product Name: TIE2

Product Clone Name: 3A5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 66-185

Epitope Target: Tie-2

Epitope Species: human

Product Reactivity/Detection: Tie-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Tie-2 Antibody (3A5): N/A

The preferred Tie-2 antibody is Tie-2 Antibody (F-6): sc-518076.