Tie-2 Antibody (F-6): sc-518076
- Tie-2 Antibody (F-6) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 65-88 within an extracellular domain of Tie-2 of human origin
- Tie-2 Antibody (F-6) is recommended for detection of Tie-2 of human origin by WB, IP, IF and ELISA
- Anti-Tie-2 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Tie-2 Antibody (F-6). This work is in progress.
QUICK LINKS
Tie-2 Antibody (F-6) is a mouse monoclonal IgM antibody that detects Tie-2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tie-2 (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tie-2, also known as Tek, is a receptor tyrosine kinase that plays a crucial role in angiogenesis and vascular stability, making Tie-2 essential for proper blood vessel formation and maintenance. Tie-2 is primarily expressed in endothelial cells and is involved in mediating the effects of angiopoietins, which are key regulators of blood vessel development and remodeling. The interaction between Tie-2 and its ligands is vital for maintaining endothelial cell integrity and preventing vascular leakage, which is particularly important in pathological conditions such as cancer and inflammatory diseases. Understanding Tie-2 function and signaling pathways can provide insights into therapeutic strategies for targeting vascular-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tie-2 Antibody (F-6) References:
- Expression of angiopoietin-1, angiopoietin-2, and the Tie-2 receptor tyrosine kinase during mouse kidney maturation. | Yuan, HT., et al. 1999. J Am Soc Nephrol. 10: 1722-36. PMID: 10446940
- Regulation of angiopoietin and Tie-2 receptor expression in non-reproductive tissues by estrogen. | Ye, F., et al. 2002. Steroids. 67: 305-10. PMID: 11856554
- Vascular endothelial growth factor modulates the Tie-2:Tie-1 receptor complex. | Tsiamis, AC., et al. 2002. Microvasc Res. 63: 149-58. PMID: 11866538
- Dual functional roles of Tie-2/angiopoietin in TNF-alpha-mediated angiogenesis. | Chen, JX., et al. 2004. Am J Physiol Heart Circ Physiol. 287: H187-95. PMID: 15210451
- Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. | Riccioni, R., et al. 2007. Stem Cells. 25: 1862-71. PMID: 17446561
- Critical role of angiopoietins/Tie-2 in hyperglycemic exacerbation of myocardial infarction and impaired angiogenesis. | Tuo, QH., et al. 2008. Am J Physiol Heart Circ Physiol. 294: H2547-57. PMID: 18408125
- Targeting the angiopoietin (Ang)/Tie-2 pathway in the crosstalk between acute myeloid leukaemia and endothelial cells: studies of Tie-2 blocking antibodies, exogenous Ang-2 and inhibition of constitutive agonistic Ang-1 release. | Reikvam, H., et al. 2010. Expert Opin Investig Drugs. 19: 169-83. PMID: 20050812
- Divergent regulation of angiopoietin-1 and -2, Tie-2, and thrombospondin-1 expression by estrogen in the baboon endometrium. | Bonagura, TW., et al. 2010. Mol Reprod Dev. 77: 430-8. PMID: 20140967
- The activity of the Ang/Tie-2 system in the brain that suffered acute carbon monoxide poisoning. | Wang, S., et al. 2013. Hum Exp Toxicol. 32: 1038-47. PMID: 23424209
- Interfering with VE-PTP stabilizes endothelial junctions in vivo via Tie-2 in the absence of VE-cadherin. | Frye, M., et al. 2015. J Exp Med. 212: 2267-87. PMID: 26642851
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tie-2 Antibody (F-6) | sc-518076 | 200 µg/ml | $322.00 | |||
Tie-2 Antibody (F-6) AC | sc-518076 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tie-2 Antibody (F-6) HRP | sc-518076 HRP | 200 µg/ml | $322.00 | |||
Tie-2 Antibody (F-6) FITC | sc-518076 FITC | 200 µg/ml | $336.00 | |||
Tie-2 Antibody (F-6) PE | sc-518076 PE | 200 µg/ml | $349.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 488 | sc-518076 AF488 | 200 µg/ml | $364.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 546 | sc-518076 AF546 | 200 µg/ml | $364.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 594 | sc-518076 AF594 | 200 µg/ml | $364.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 647 | sc-518076 AF647 | 200 µg/ml | $364.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 680 | sc-518076 AF680 | 200 µg/ml | $364.00 | |||
Tie-2 Antibody (F-6) Alexa Fluor® 790 | sc-518076 AF790 | 200 µg/ml | $364.00 |