TBX5 Antibody (A-4): sc-376952

TBX5 Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects TBX5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TBX5 plays a crucial role in early heart development, and proper function is essential for the formation of the heart and upper limbs. TBX5 is primarily located in the nucleus, where TBX5 acts as a transcription factor, regulating the expression of genes involved in cardiac morphogenesis. The significance of TBX5′s nuclear localization lies in TBX5′s ability to interact with other transcription factors and co-factors, thereby influencing the developmental pathways that lead to the formation of the heart and limbs. Mutations in TBX5 can lead to congenital heart defects and upper limb malformations, as seen in Holt-Oram syndrome, highlighting TBX5′s importance in both normal development and disease. Anti-TBX5 antibody (A-4) is an invaluable tool for researchers studying cardiac development and related congenital anomalies, providing insights into the molecular mechanisms underlying these critical processes.

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TBX5 Antibody (A-4) References:

1. Developmental structure-function insights from Tbx5(del/+) mouse model of Holt-Oram syndrome.  |  Keller, BB. 2005. Am J Physiol Heart Circ Physiol. 289: H975-6. PMID: 16100253
2. Molecular basis of the clinical features of Holt-Oram syndrome resulting from missense and extended protein mutations of the TBX5 gene as well as TBX5 intragenic duplications.  |  Al-Qattan, MM. and Abou Al-Shaar, H. 2015. Gene. 560: 129-36. PMID: 25680289
3. Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease.  |  Kathiriya, IS., et al. 2021. Dev Cell. 56: 292-309.e9. PMID: 33321106
4. Tbx5 variants disrupt Nav1.5 function differently in patients diagnosed with Brugada or Long QT Syndrome.  |  Nieto-Marín, P., et al. 2022. Cardiovasc Res. 118: 1046-1060. PMID: 33576403
5. Tbx5 drives Aldh1a2 expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development.  |  Rankin, SA., et al. 2021. Elife. 10: PMID: 34643182
6. Patient-Specific TBX5-G125R Variant Induces Profound Transcriptional Deregulation and Atrial Dysfunction.  |  van Ouwerkerk, AF., et al. 2022. Circulation. 145: 606-619. PMID: 35113653
7. An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility.  |  Bosada, FM., et al. 2023. Elife. 12: PMID: 36715501
8. A Genomic Link From Heart Failure to Atrial Fibrillation Risk: FOG2 Modulates a TBX5/GATA4-Dependent Atrial Gene Regulatory Network.  |  Broman, MT., et al. 2024. Circulation. 149: 1205-1230. PMID: 38189150
9. Tbx5 maintains atrial identity in post-natal cardiomyocytes by regulating an atrial-specific enhancer network.  |  Sweat, ME., et al. 2023. Nat Cardiovasc Res. 2: 881-898. PMID: 38344303
10. Overexpression of transcription factor TBX5 inhibits the activation of YAP1-TEAD1 pathway to promote ferroptosis in lung cancer cells.  |  Ma, R., et al. 2024. Biochem Biophys Res Commun. 718: 150037. PMID: 38735135

The preferred TBX5 antibody is TBX5 Antibody (A-6): sc-515536.