TBX5 Antibody (A-6): sc-515536

TBX5 Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects TBX5 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TBX5 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TBX5 plays a crucial role in cardiac and limb development, as TBX5 is essential for proper heart and upper limb formation during embryogenesis. Mutations in TBX5 are linked to Holt-Oram syndrome, which manifests as congenital heart defects and upper limb malformations, highlighting TBX5′s importance in both cardiac and skeletal development. Additionally, TBX5 interacts with other transcription factors, such as TBX20, to regulate gene expression necessary for heart morphogenesis, underscoring TBX5′s significance in developmental biology and potential implications in congenital heart disease research. The conserved T-box domain within the Tbx gene family facilitates DNA-binding and protein dimerization, which are critical for transcriptional regulation of target genes involved in various developmental processes, making TBX5 a vital component in understanding both normal and pathological development.

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TBX5 Antibody (A-6) References:

1. Transcription repression by Xenopus ET and its human ortholog TBX3, a gene involved in ulnar-mammary syndrome.  |  He, Ml., et al. 1999. Proc Natl Acad Sci U S A. 96: 10212-7. PMID: 10468588
2. Developmental regulation of Tbx5 in zebrafish embryogenesis.  |  Begemann, G. and Ingham, PW. 2000. Mech Dev. 90: 299-304. PMID: 10640716
3. tbx20, a new vertebrate T-box gene expressed in the cranial motor neurons and developing cardiovascular structures in zebrafish.  |  Ahn, DG., et al. 2000. Mech Dev. 95: 253-8. PMID: 10906473
4. The comparative genomics of T-box genes.  |  Minguillon, C. and Logan, M. 2003. Brief Funct Genomic Proteomic. 2: 224-33. PMID: 15239925
5. Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis.  |  Brown, DD., et al. 2005. Development. 132: 553-63. PMID: 15634698
6. Molecular genetic and ocular findings in patients with holt-oram syndrome.  |  Gruenauer-Kloevekorn, C., et al. 2005. Ophthalmic Genet. 26: 1-8. PMID: 15823919
7. TBX5 genetic testing validates strict clinical criteria for Holt-Oram syndrome.  |  McDermott, DA., et al. 2005. Pediatr Res. 58: 981-6. PMID: 16183809
8. A WW domain protein TAZ is a critical coactivator for TBX5, a transcription factor implicated in Holt-Oram syndrome.  |  Murakami, M., et al. 2005. Proc Natl Acad Sci U S A. 102: 18034-9. PMID: 16332960
9. TBX5 loss-of-function mutation contributes to familial dilated cardiomyopathy.  |  Zhang, XL., et al. 2015. Biochem Biophys Res Commun. 459: 166-71. PMID: 25725155
10. Evolutionarily conserved Tbx5-Wnt2/2b pathway orchestrates cardiopulmonary development.  |  Steimle, JD., et al. 2018. Proc Natl Acad Sci U S A. 115: E10615-E10624. PMID: 30352852
11. Preclinical evidence for the therapeutic value of TBX5 normalization in arrhythmia control.  |  Rathjens, FS., et al. 2021. Cardiovasc Res. 117: 1908-1922. PMID: 32777030
12. Cloning, mapping, and expression analysis of TBX15, a new member of the T-Box gene family.  |  Agulnik, SI., et al. 1998. Genomics. 51: 68-75. PMID: 9693034

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TBX5 Antibody (A-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-TBX5 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TBX5 Antibody (A-6) Specifications

Alternate Product Name: T-box transcription factor 5

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 269-292

Epitope Target: TBX5

Epitope Species: human

Product Reactivity/Detection: TBX5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for TBX5 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent