SPARC Antibody (AON-5031): sc-73472

SPARC Antibody (AON-5031) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPARC in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-SPARC antibody (AON-5031) is available in a non-conjugated form, allowing for versatile experimental setups. SPARC, or secreted protein acidic and rich in cysteine, is a phosphorylated, acidic, glycine-rich glycoprotein that plays a crucial role in various biological processes, particularly in bone metabolism and tissue remodeling. SPARC is predominantly secreted by endothelial cells and is found in significant quantities in the parietal endoderm of mouse embryos and in the human placenta. SPARC is identical to osteonectin, a highly conserved protein essential for bone calcification, underscoring its importance in skeletal health. SPARC′s structure, characterized by its unique glycoprotein composition, allows interaction with various extracellular matrix components and growth factors, facilitating cellular signaling and influencing cell behavior. This structural versatility is vital for regulating endothelial barrier function, as SPARC induces changes in cell shape and promotes the formation of intercellular gaps, which are critical for the paracellular transport of macromolecules during physiological and pathological processes.

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SPARC Antibody (AON-5031) References:

1. Role of SPARC in bone remodeling and cancer-related bone metastasis.  |  Ribeiro, N., et al. 2014. J Cell Biochem. 115: 17-26. PMID: 24038053
2. SPARC in Tumor Pathophysiology and as a Potential Therapeutic Target.  |  Feng, J. and Tang, L. 2014. Curr Pharm Des. 20: 6182-90. PMID: 24947586
3. Roles of SPARC in urothelial carcinogenesis, progression and metastasis.  |  Said, N. 2016. Oncotarget. 7: 67574-67585. PMID: 27564266
4. Isolation of the osteonectin gene: evidence that a variable region of the osteonectin molecule is encoded within one exon.  |  Findlay, DM., et al. 1988. Biochemistry. 27: 1483-9. PMID: 2835093
5. Macrophage-derived SPARC Attenuates M2-mediated Pro-tumour Phenotypes.  |  Hu, J., et al. 2020. J Cancer. 11: 2981-2992. PMID: 32226513
6. SPARC in hematologic malignancies and novel technique for hematological disease with its abnormal expression.  |  Nian, Q., et al. 2022. Biomed Pharmacother. 153: 113519. PMID: 36076604
7. GP60 and SPARC as albumin receptors: key targeted sites for the delivery of antitumor drugs.  |  Ji, Q., et al. 2024. Front Pharmacol. 15: 1329636. PMID: 38323081
8. Immunohistochemical expression of SPARC in odontogenic keratocysts: a comparative study with other odontogenic cysts.  |  Poomsawat, S., et al. 2024. BMC Oral Health. 24: 223. PMID: 38347494
9. SPARC activates p38γ signaling to promote PFKFB3 protein stabilization and contributes to keloid fibroblast glycolysis.  |  Liu, Y., et al. 2024. Inflamm Regen. 44: 44. PMID: 39482755
10. Osteonectin, a bone-specific protein linking mineral to collagen.  |  Termine, JD., et al. 1981. Cell. 26: 99-105. PMID: 7034958
11. SPARC (secreted protein acidic and rich in cysteine) regulates endothelial cell shape and barrier function.  |  Goldblum, SE., et al. 1994. Proc Natl Acad Sci U S A. 91: 3448-52. PMID: 8159767
12. Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.  |  Le Beau, MM., et al. 1993. Proc Natl Acad Sci U S A. 90: 5484-8. PMID: 8516290

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPARC Antibody (AON-5031) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-SPARC Antibody (AON-5031) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPARC Antibody (AON-5031) Specifications

Alternate Product Name: SPARC

Product Clone Name: AON-5031

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SPARC

Epitope Species: human

Product Reactivity/Detection: bone SPARC and platelet SPARC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 5% glycerol

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for SPARC Antibody (AON-5031): N/A

The preferred SPARC antibody is SPARC Antibody (AON-1): sc-33645.