Smad8 Antibody (A-1): sc-518051
- Smad8 Antibody (A-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against a GST fusion protein corresponding to amino acids 45-64 representing full length Smad8 of human origin
- Anti-Smad8 Antibody (A-1) is recommended for detection of Smad8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Smad8 Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Smad8 Antibody (A-1) for WB applications. These reagents are now offered in bundles with Smad8 Antibody (A-1) (see ordering information below).
Smad8 Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Smad8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Smad8 antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smad8, also known as Smad9 or MADH6, plays a crucial role in the transforming growth factor-beta (TGFβ) and bone morphogenetic protein (BMP) signaling pathways, acting as a downstream effector that mediates cellular responses to these important growth factors. Smad8 is primarily located in the nucleus, where Smad8 translocates upon activation by TGFβ or BMP ligands, allowing regulation of gene expression vital for processes such as cell differentiation, proliferation, and apoptosis. This nuclear localization enables Smad8 to interact with other transcription factors and co-regulators, thereby influencing the transcriptional landscape in response to extracellular signals. Understanding the function and localization of Smad8 is important for elucidating Smad8′s role in various physiological and pathological processes, including development, immune response, and cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Smad8 Antibody (A-1) References:
- Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury. | Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
- Smads and chromatin modulation. | van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
- TGF-beta signalling through the Smad pathway. | Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
- A human Mad protein acting as a BMP-regulated transcriptional activator. | Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
- MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. | Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
- Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. | Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
- Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways. | Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
- Smad6 inhibits signalling by the TGF-beta superfamily. | Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
- Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase. | Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
- TGF-beta signalling from cell membrane to nucleus through SMAD proteins. | Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad8 Antibody (A-1) | sc-518051 | 200 µg/ml | $322.00 | |||
Smad8 Antibody (A-1): m-IgG Fc BP-HRP Bundle | sc-528084 | 200 µg Ab; 10 µg BP | $361.00 | |||
Smad8 Antibody (A-1): m-IgG1 BP-HRP Bundle | sc-533457 | 200 µg Ab; 20 µg BP | $361.00 | |||
Smad8 Antibody (A-1) AC | sc-518051 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Smad8 Antibody (A-1) HRP | sc-518051 HRP | 200 µg/ml | $322.00 | |||
Smad8 Antibody (A-1) FITC | sc-518051 FITC | 200 µg/ml | $336.00 | |||
Smad8 Antibody (A-1) PE | sc-518051 PE | 200 µg/ml | $349.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 488 | sc-518051 AF488 | 200 µg/ml | $364.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 546 | sc-518051 AF546 | 200 µg/ml | $364.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 594 | sc-518051 AF594 | 200 µg/ml | $364.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 647 | sc-518051 AF647 | 200 µg/ml | $364.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 680 | sc-518051 AF680 | 200 µg/ml | $364.00 | |||
Smad8 Antibody (A-1) Alexa Fluor® 790 | sc-518051 AF790 | 200 µg/ml | $364.00 |