Smad6 Antibody (D-4): sc-25321

Smad6 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Smad6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Smad6 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smad6 protein plays a crucial role in regulating TGFβ/BMP signaling pathways, acting as a negative regulator that inhibits phosphorylation of other Smad proteins, thereby modulating cellular responses to growth factors. This function helps maintain cellular homeostasis and prevents excessive signaling that could lead to pathological conditions such as fibrosis or cancer. Through interference with Smad2 and Smad3 activation, Smad6 ensures a balanced response to TGFβ and activin, which are vital for various developmental processes and tissue homeostasis. Smad6′s interaction with other Smad family members highlights its significance in the intricate network of signaling pathways that govern cellular behavior, making Smad6 (D-4) monoclonal antibody an essential tool for researchers studying these critical biological processes.

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Smad6 Antibody (D-4) References:

1. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury.  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
2. Smads and chromatin modulation.  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
3. TGF-beta signalling through the Smad pathway.  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
4. SMAD6-deficiency in human genetic disorders.  |  Luyckx, I., et al. 2022. NPJ Genom Med. 7: 68. PMID: 36414630
5. SMAD6 variants in nonsyndromic craniosynostosis.  |  Timberlake, AT. 2023. Eur J Hum Genet. 31: 611-612. PMID: 36797468
6. A human Mad protein acting as a BMP-regulated transcriptional activator.  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
7. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
8. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
9. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
10. Smad6 inhibits signalling by the TGF-beta superfamily.  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
11. Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase.  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
12. TGF-beta signalling from cell membrane to nucleus through SMAD proteins.  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smad6 Antibody (D-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Smad6 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smad6 Antibody (D-4) Specifications

Alternate Product Name: Smad6

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 41-190

Epitope Target: Smad6

Epitope Species: human

Product Reactivity/Detection: Smad6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Smad6 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent