Smad1 Antibody (A-4): sc-7965

Smad1 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody raised against the full-length Smad1 protein of human origin, reactive with mouse, rat, mink, and human species. Smad1, also known as Mothers against decapentaplegic homolog 1 (MADH1), MADR1, or JV4-1, is a critical intracellular signal transducer in the Bone Morphogenetic Protein (BMP) signaling pathway, which is part of the Transforming Growth Factor-beta (TGF-β) superfamily. Upon phosphorylation by BMP type I receptors, Smad1 forms a complex with Smad4 and translocates to the nucleus to regulate the transcription of genes involved in cell proliferation, differentiation, and apoptosis. This function is essential for processes such as bone formation, neuronal development, and embryogenesis. Smad1 contains two conserved domains, MH1 and MH2, which are important for DNA binding and protein interactions, respectively; these structural features are crucial for its role in transcriptional regulation. Dysregulation of Smad1 signaling can lead to developmental abnormalities and has been implicated in various diseases, including cancer and fibrosis. Smad1 monoclonal antibody (A-4) is suitable for applications like western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC) on paraffin-embedded sections, and enzyme-linked immunosorbent assay (ELISA), making Smad1 monoclonal antibody (A-4) a valuable tool for studying Smad1′s role in cellular signaling pathways. By targeting Smad1, researchers can investigate the molecular mechanisms of BMP signaling and its impact on development and disease.

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Smad1 Antibody (A-4) References:

1. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury.  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
2. Smads and chromatin modulation.  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
3. TGF-beta signalling through the Smad pathway.  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
4. A human Mad protein acting as a BMP-regulated transcriptional activator.  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
5. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
6. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
7. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
8. Smad6 inhibits signalling by the TGF-beta superfamily.  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
9. Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase.  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
10. TGF-beta signalling from cell membrane to nucleus through SMAD proteins.  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smad1 Antibody (A-4) has 112 citations in a variety of scientific publications.

Primary antibodies like Anti-Smad1 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smad1 Antibody (A-4) Specifications

Alternate Product Name: Smad1

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-465

Epitope Target: Smad1

Epitope Species: human

Product Reactivity/Detection: Smad1

Species Reactivity/Detection: mouse, rat, human, mink

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 112 citations in various scientific publications

Available Conjugates/Forms for Smad1 Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Smad1/2/3 antibody is Smad1/2/3 Antibody (H-2): sc-7960.