SIRT5 Antibody (G-2): sc-271635

SIRT5 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects SIRT5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SIRT5 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIRT5, also known as NAD-dependent deacetylase sirtuin-5 or SIR2L5, is a 310 amino acid protein belonging to the class III sirtuins, which are crucial for regulating cellular processes through deacetylase activity. SIRT5 is primarily localized in mitochondria, where SIRT5 plays a significant role in metabolic regulation and may influence aging processes in humans. Mitochondrial localization of SIRT5 enables direct interaction with various metabolic pathways, including fatty acid oxidation and urea cycle. SIRT5 deacetylates specific mitochondrial proteins, modulating their activity and contributing to overall metabolic homeostasis of cells. Suramin can inhibit SIRT5 by disrupting growth factor binding, highlighting SIRT5′s potential as a therapeutic target in metabolic disorders. Two SIRT5 isoforms arise from alternative splicing, further diversifying functional roles within cells.

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SIRT5 Antibody (G-2) References:

1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.  |  Frye, RA. 1999. Biochem Biophys Res Commun. 260: 273-9. PMID: 10381378
2. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.  |  Frye, RA. 2000. Biochem Biophys Res Commun. 273: 793-8. PMID: 10873683
3. Differential regulation of the Sir2 histone deacetylase gene family by inhibitors of class I and II histone deacetylases.  |  Kyrylenko, S., et al. 2003. Cell Mol Life Sci. 60: 1990-7. PMID: 14523559
4. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.  |  Michishita, E., et al. 2005. Mol Biol Cell. 16: 4623-35. PMID: 16079181
5. Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization.  |  Mahlknecht, U., et al. 2006. Cytogenet Genome Res. 112: 208-12. PMID: 16484774
6. DNA pooling: a comprehensive, multi-stage association analysis of ACSL6 and SIRT5 polymorphisms in schizophrenia.  |  Chowdari, KV., et al. 2007. Genes Brain Behav. 6: 229-39. PMID: 16827919
7. Structural basis of inhibition of the human NAD+-dependent deacetylase SIRT5 by suramin.  |  Schuetz, A., et al. 2007. Structure. 15: 377-89. PMID: 17355872
8. Localization of mouse mitochondrial SIRT proteins: shift of SIRT3 to nucleus by co-expression with SIRT5.  |  Nakamura, Y., et al. 2008. Biochem Biophys Res Commun. 366: 174-9. PMID: 18054327
9. SIRT5 regulation of ammonia-induced autophagy and mitophagy.  |  Polletta, L., et al. 2015. Autophagy. 11: 253-70. PMID: 25700560
10. Loss of SIRT5 promotes bile acid-induced immunosuppressive microenvironment and hepatocarcinogenesis.  |  Sun, R., et al. 2022. J Hepatol. 77: 453-466. PMID: 35292350
11. SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis.  |  Mao, J., et al. 2023. Exp Mol Med. 55: 253-268. PMID: 36653443
12. SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration.  |  Teng, P., et al. 2024. Cell Death Differ. 31: 65-77. PMID: 38007551

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRT5 Antibody (G-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRT5 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRT5 Antibody (G-2) Specifications

Alternate Product Name: SIRT5

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-310

Epitope Target: SIRT5

Epitope Species: human

Product Reactivity/Detection: SIRT5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for SIRT5 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin