SIRT5 Antibody (G-2) recommended for detection of SIRT5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SIRT5 Antibody (G-2): sc-271635

SIRT5 Antibody (G-2) is rated 4.1 out of 5 by 10.
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Datasheets

    • Anti-SIRT5 Antibody (G-2) is a mouse monoclonal IgG1 (kappa light chain) SIRT5 antibody provided at 200 µg/ml
    • raised against amino acids 1-310 representing full length SIRT5 of human origin
    • Anti-SIRT5 Antibody (G-2) is recommended for detection of SIRT5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SIRT5 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRT5 (G-2): sc-271635.

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-20

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-19
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Rated 2 out of 5 by from 小鼠肾脏的免疫组化 使用贵公司抗体做了C57小鼠肾脏的免疫组化,在1:50稀释比例下,阳性对照组及正常小鼠肾脏的染色弱不明显。
Date published: 2017-10-16
Rated 4 out of 5 by from specific band I have done knocking down,and the band is specific.
Date published: 2017-07-10
Rated 5 out of 5 by from good reactivity WB on mouse brain tissue, diluted with 1:1000, the band is very clear.
Date published: 2017-05-23
Rated 5 out of 5 by from Good for WB I tested this antibodies on mouse primary hepatocytes, and they showed nice band.
Date published: 2017-02-13
Rated 5 out of 5 by from Excellent! This antibody works great western blot analysis of SIRT5 expression in PC-12, Jurkat and MOLT-4 whole cell lysates and human fetal liver, mouse kidney and mouse brain tissue extracts.
Date published: 2017-01-12
Rated 1 out of 5 by from Not good for IF Tried using this antibody for IF at several concentrations and there was no signal.
Date published: 2016-12-13
Rated 4 out of 5 by from Good antibody The antibody is good and can be used well !!!
Date published: 2016-11-10
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-30
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