SAP 145 Antibody (D-4): sc-514976

SAP 145 Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects SAP 145 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAP 145 is a crucial component of the SF3b complex, which is integral to the assembly of the spliceosome, a vital molecular machine responsible for the splicing of pre-mRNA. SAP 145 (D-4) antibody recognizes a protein that interacts with other spliceosomal proteins, including SAP 49, SAP 130, and SAP 155, facilitating the proper assembly and function of the spliceosome. Notably, SAP 145 directly interacts with SAP 49, a relationship that is essential for the stability and functionality of the spliceosomal complex. The interaction between these proteins is particularly significant as SAP 49′s amino-terminus contains two RNA-recognition motifs that are critical for binding to both U2 small nuclear ribonucleoprotein and pre-mRNA. This interaction not only highlights SAP 145′s role in spliceosome assembly but also emphasizes SAP 145′s potential impact on gene expression regulation, making SAP 145 a key player in cellular processes. Anti-SAP 145 antibody (D-4) serves as an invaluable tool for researchers studying RNA splicing and related pathways.

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SAP 145 Antibody (D-4) References:

1. Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA.  |  Pauling, MH., et al. 2000. Mol Cell Biol. 20: 2176-85. PMID: 10688664
2. Spermatogenetic expression of RNA-binding motif protein 7, a protein that interacts with splicing factors.  |  Guo, TB., et al. 2003. J Androl. 24: 204-14. PMID: 12634307
3. Proteomic characterization of protein phosphatase complexes of the mammalian nucleus.  |  Tran, HT., et al. 2004. Mol Cell Proteomics. 3: 257-65. PMID: 14724321
4. Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145.  |  Terada, Y. and Yasuda, Y. 2006. Mol Cell Biol. 26: 8149-58. PMID: 16923959
5. Human immunodeficiency virus type 1 Vpr interacts with spliceosomal protein SAP145 to mediate cellular pre-mRNA splicing inhibition.  |  Hashizume, C., et al. 2007. Microbes Infect. 9: 490-7. PMID: 17347016
6. Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2.  |  Hadjikyriacou, A., et al. 2015. J Biol Chem. 290: 16723-43. PMID: 25979344
7. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.  |  Champion-Arnaud, P. and Reed, R. 1994. Genes Dev. 8: 1974-83. PMID: 7958871
8. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.  |  Wells, SE., et al. 1996. Genes Dev. 10: 220-32. PMID: 8566755
9. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A.  |  Gozani, O., et al. 1996. Genes Dev. 10: 233-43. PMID: 8566756
10. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.  |  Seghezzi, W., et al. 1998. Mol Cell Biol. 18: 4526-36. PMID: 9671462

The preferred SAP 145 antibody is SAP 145 Antibody (C-12): sc-514930.