SAP 145 Antibody (C-12): sc-514930

SAP 145 Antibody (C-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects SAP 145 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SAP 145 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAP 145 protein is a crucial component of the SF3b complex, which is associated with the U2 small nuclear ribonucleoprotein (snRNP) and plays an essential role in spliceosome assembly. This complex is vital for the splicing of pre-mRNA, a process that is critical for the proper expression of genes. The interaction of SAP 145 with other spliceosomal proteins, such as SAP 49, SAP 130, and SAP 155, highlights SAP 145′s importance in the formation and stability of the spliceosome. Notably, the direct interaction between SAP 49 and SAP 145 is mediated by the amino-terminus of SAP 49, which contains two RNA-recognition motifs, suggesting this interaction is integral to the complex′s ability to tether U2 snRNP to the branch site during splicing. Anti-SAP 145 antibody (C-12) effectively detects and characterizes SAP 145 protein, making SAP 145 (C-12) monoclonal antibody an invaluable tool for researchers studying RNA processing and gene regulation.

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SAP 145 Antibody (C-12) References:

1. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP.  |  Krämer, A., et al. 1999. J Cell Biol. 145: 1355-68. PMID: 10385517
2. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.  |  Das, BK., et al. 1999. Mol Cell Biol. 19: 6796-802. PMID: 10490618
3. Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step.  |  Bryant, HE., et al. 2001. J Virol. 75: 4376-85. PMID: 11287586
4. DRBD1 is the Trypanosoma brucei homologue of the spliceosome-associated protein 49.  |  Manful, T., et al. 2009. Mol Biochem Parasitol. 166: 186-9. PMID: 19450735
5. PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145.  |  Yang, Y., et al. 2015. Nat Commun. 6: 6428. PMID: 25737013
6. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.  |  Falk, S., et al. 2016. Nat Commun. 7: 13573. PMID: 27905398
7. Chemical probes for protein arginine methyltransferases.  |  Li, ASM., et al. 2020. Methods. 175: 30-43. PMID: 31809836
8. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.  |  Champion-Arnaud, P. and Reed, R. 1994. Genes Dev. 8: 1974-83. PMID: 7958871
9. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.  |  Wells, SE., et al. 1996. Genes Dev. 10: 220-32. PMID: 8566755
10. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A.  |  Gozani, O., et al. 1996. Genes Dev. 10: 233-43. PMID: 8566756
11. Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits.  |  Igel, H., et al. 1998. RNA. 4: 1-10. PMID: 9436903
12. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.  |  Seghezzi, W., et al. 1998. Mol Cell Biol. 18: 4526-36. PMID: 9671462

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SAP 145 Antibody (C-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SAP 145 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SAP 145 Antibody (C-12) Specifications

Alternate Product Name: SAP 145

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 596-895

Epitope Target: SAP 145

Epitope Species: human

Product Reactivity/Detection: SAP 145

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SAP 145 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin